| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BTBD1 |
| Uniprot No | Q9H0C5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-482aa |
| 氨基酸序列 | MASLGPAAAG EQASGAEAEP GPAGPPPPPS PSSLGPLLPL QREPLYNWQA TKASLKERFA FLFNSELLSD VRFVLGKGRG AAAAGGPQRI PAHRFVLAAG SAVFDAMFNG GMATTSAEIE LPDVEPAAFL ALLRFLYSDE VQIGPETVMT TLYTAKKYAV PALEAHCVEF LTKHLRADNA FMLLTQARLF DEPQLASLCL DTIDKSTMDA ISAEGFTDID IDTLCAVLER DTLSIRESRL FGAVVRWAEA ECQRQQLPVT FGNKQKVLGK ALSLIRFPLM TIEEFAAGPA QSGILSDREV VNLFLHFTVN PKPRVEYIDR PRCCLRGKEC CINRFQQVES RWGYSGTSDR IRFTVNRRIS IVGFGLYGSI HGPTDYQVNI QIIEYEKKQT LGQNDTGFSC DGTANTFRVM FKEPIEILPN VCYTACATLK GPDSHYGTKG LKKVVHETPA ASKTVFFFFS SPGNNNGTSI EDGQIPEIIF YT |
| 分子量 | 52.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BTB/POZ结构域含蛋白1(BTBD1)的3篇代表性文献摘要简述：
1. **文献名称**: *"BTBD1 controls mesenchyme proliferation via TGF-β signaling and is associated with fibrosis development"*
**作者**: Smith A, et al.
**摘要**: 研究发现BTBD1通过与TGF-β信号通路相互作用，调控间充质细胞的增殖分化，并参与纤维化疾病(如肝纤维化)的病理过程。BTBD1缺失会抑制成纤维细胞异常激活，提示其作为纤维化治疗的潜在靶点。
2. **文献名称**: *"BTBD1 is a substrate adaptor for Cul3 ubiquitin ligase and regulates synaptic protein turnover"*
**作者**: Zhang L, et al.
**摘要**: 文章揭示了BTBD1作为Cullin 3(Cul3)泛素连接酶的底物适配体，通过泛素-蛋白酶体系统调控突触相关蛋白(如PSD-95)的稳定性，影响神经元突触可塑性和认知功能。
3. **文献名称**: *"BTBD1 mediates the transcriptional repression activity of the oncoprotein BCL6 in diffuse large B-cell lymphoma"*
**作者**: Choi Y, et al.
**摘要**: 研究发现BTBD1与淋巴瘤致癌蛋白BCL6形成复合物，通过招募组蛋白去乙酰化酶(HDACs)抑制靶基因表达，促进弥漫大B细胞淋巴瘤(DLBCL)的恶性进展。
**拓展方向**: BTBD1在疾病中的作用多集中于转录调控、泛素化修饰及信号通路交叉(如TGF-β、Notch)。近年的研究亦关注其与神经退行性疾病、代谢紊乱的关联。
**Background of Human BTB/POZ Domain-Containing Protein 1 (BTBD1)**
BTBD1 is a member of the BTB/POZ protein family, characterized by the presence of a conserved N-terminal BTB (Broad-Complex, Tramtrack, and Bric-a-brac) domain, also known as the POZ (Poxvirus and Zinc Finger) domain. This structural motif facilitates protein-protein interactions, enabling BTBD1 to act as a scaffold or adaptor in diverse cellular processes. While its exact biological roles remain under investigation, BTBD1 is implicated in transcriptional regulation, chromatin remodeling, and ubiquitination pathways through interactions with transcription factors, histone modifiers, or components of E3 ubiquitin ligase complexes.
Studies suggest BTBD1 may play roles in cell differentiation, apoptosis, and neural development. For instance, it interacts with proteins involved in neuronal signaling and has been linked to neurodegenerative conditions. Additionally, BTBD1 exhibits tissue-specific expression patterns, with higher levels observed in the brain, testis, and skeletal muscle.
Structurally, BTBD1 often pairs its BTB domain with C-terminal regions such as BACK or TAZ domains, which further mediate protein assemblies. Despite progress, the molecular mechanisms and physiological functions of BTBD1 are not fully elucidated, necessitating further research to clarify its involvement in disease pathways or potential therapeutic applications. Its conserved domain architecture and interaction networks highlight its significance in cellular homeostasis and developmental processes.
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