| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BTBD14A |
| Uniprot No | Q96BF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 氨基酸序列 | MSQMLHIEIPNFGNTVLGCLNEQRLLGLYCDVSIVVKGQAFKAHRAVLAASSLYFRDLFSGNSKSAFELPGSVPPACFQQILSFCYTGRLTMTASEQLVVMYTAGFLQIQHIVERGTDLMFKVSSPHCDSQTAVIEDAGSEPQSPCNQLQPAAAAAAPYVVSPSVPIPLLTRVKHEAMELPPAGPGLAPKRPLETGPRDGVAVAAGAAVAAGTAPLKLPRVSYYGVPSLATLIPGIQQMPYPQGERTSPGASSLPTTDSPTSYHNEEDEEDDEAYDTMVEEQYGQMYIKASGSYAVQEKPEPVPLESRSCVLIRRDLVALPASLISQIGYRCHPKLYSEGDPGEKLELVAGSGVYITRGQLMNCHLCAGVKHKVLLRRLLATFFDRNTLANSCGTGIRSSTSDPSRKPLDSRVLNAVKLYCQNFAPSFKESEMNVIAADMCTNARRVRKRWLPKIKSMLPEGVEMYRTVMGSAAASVPLDPEFPPAAAQVFEQRIYAERRGDAATIVALRTDAVNVDLSAAANPAFDAGEEVDGAGSVIQEVAAPEPLPADGQSPPQPFEQGGGGPSRPQTPAAAARRPEGTYAGTL |
| 分子量 | 89.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条假设性的参考文献示例(注：基于现有知识虚构，建议通过学术数据库核实真实文献)：
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1. **标题**：Structural and functional characterization of the BTBD14A-HDAC complex in transcriptional regulation
**作者**：Takaseu H, et al.
**摘要**：本研究解析了重组人BTBD14A蛋白与组蛋白去乙酰化酶(HDAC)的相互作用，揭示其通过招募HDAC复合体调控靶基因的表观遗传修饰，影响细胞分化进程。
2. **标题**：BTBD14A acts as a substrate adaptor in a Cul3 ubiquitin ligase complex
**作者**：Lee S, Kim J.
**摘要**：通过重组蛋白实验，发现BTBD14A的BTB结构域介导其与Cul3泛素连接酶结合，促进特定底物的泛素化降解，提示其在蛋白质稳态中的调控作用。
3. **标题**：BTBD14A suppresses breast cancer metastasis via modulating cytoskeletal dynamics
**作者**：Wang L, et al.
**摘要**：利用重组BTBD14A进行的功能研究表明，该蛋白通过抑制Rho GTPase信号通路降低乳腺癌细胞侵袭和迁移能力，可能作为潜在治疗靶点。
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**提示**：实际文献可能较少集中在BTBD14A，建议扩展检索其别名(如C3orf13)或相关通路(如染色质重塑、癌症转移)。使用PubMed或Google Scholar以“BTBD14A recombinant protein”或“BTBD14A function”为关键词搜索最新研究。
**Background of Recombinant Human BTBD14A Protein**
BTBD14A (BTB/POZ domain-containing protein 14A), also known as BANP or SMAP, is a nuclear protein encoded by the *BTBD14A* gene. It belongs to the BTB/POZ protein family, characterized by a conserved BTB (Bric-a-brac, Tramtrack, Broad Complex) domain implicated in protein-protein interactions and transcriptional regulation. BTBD14A has gained attention for its potential roles in chromatin remodeling, gene expression modulation, and cellular processes like cell cycle control and DNA damage response.
Structurally, BTBD14A contains distinct domains, including the BTB/POZ domain for scaffolding and a C-terminal region that may mediate DNA or protein binding. Studies suggest it acts as a transcriptional repressor by binding to specific promoter regions, particularly those with CGCG motifs, and recruiting chromatin-modifying complexes. Notably, BTBD14A is reported to regulate genes involved in tumor suppression, neuronal development, and metabolic pathways, linking it to diseases such as cancer (e.g., glioblastoma, colorectal cancer) and neurodegeneration.
Recombinant BTBD14A protein is engineered for *in vitro* studies to dissect its molecular functions, interactions, and regulatory mechanisms. Produced typically in *E. coli* or mammalian expression systems, it enables biochemical assays, structural analyses, and drug discovery efforts. Despite emerging interest, many aspects of BTBD14A’s biology, including its precise targets and pathological relevance, remain underexplored, necessitating further research to unveil its therapeutic potential.
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