| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSPRY |
| Uniprot No | Q5W0U4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MSAEGAEPGP GSGSGPGPGP LCPEHGQALS WFCGSERRPV CAACAGLGGR CRGHRIRRAE ERAEELRNKI VDQCERLQLQ SAAITKYVAD VLPGKNQRAV SMASAARELV IQRLSLVRSL CESEEQRLLE QVHGEEERAH QSILTQRVHW AEALQKLDTI RTGLVGMLTH LDDLQLIQKE QEIFERTEEA EGILDPQESE MLNFNEKCTR SPLLTQLWAT AVLGSLSGTE DIRIDERTVS PFLQLSDDRK TLTFSTKKSK ACADGPERFD HWPNALAATS FQNGLHAWMV NVQNSCAYKV GVASGHLPRK GSGSDCRLGH NAFSWVFSRY DQEFRFSHNG QHEPLGLLRG PAQLGVVLDL QVQELLFYEP ASGTVLCAHH VSFPGPLFPV FAVADQTISI VR |
| 分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BSPRY蛋白的3-4篇典型参考文献及其内容概括：
1. **文献名称**：*BSPRY is a negative regulator of the EGFR signaling pathway in colorectal cancer*
**作者**：Wang Y, et al. (2018)
**摘要**：研究发现BSPRY通过抑制EGFR-MAPK信号通路，在结直肠癌细胞中发挥抑癌作用，降低肿瘤细胞增殖和迁移能力。
2. **文献名称**：*Structural insights into the B-box and SPRY domains of BSPRY protein*
**作者**：Gregory MF, et al. (2015)
**摘要**：通过X射线晶体学和NMR分析，揭示了BSPRY的B-box和SPRY结构域的三维结构，提出其对蛋白质相互作用的调控机制。
3. **文献名称**：*BSPRY interacts with TRPV5/6 to regulate calcium homeostasis in renal cells*
**作者**：Hoenderop JG, et al. (2010)
**摘要**：该研究证实BSPRY与肾上皮细胞中TRPV5/6钙离子通道结合，影响钙离子再吸收，提示其在钙代谢疾病中的潜在作用。
4. **文献名称**：*The role of BSPRY in prostate cancer progression and androgen receptor signaling*
**作者**：Chen Z, et al. (2021)
**摘要**：阐明了BSPRY在前列腺癌中通过干扰雄激素受体(AR)核转运抑制肿瘤生长的分子机制，为靶向治疗提供新思路。
**备注**：以上文献信息为模拟示例，实际研究中需根据具体需求查阅真实文献数据库(如PubMed)。
BSPRY (B-box and SPRY Domain-Containing Protein) is a member of the SPRY domain protein family, characterized by the presence of B-box and SPRY domains. The B-box is a zinc-binding motif involved in protein-protein interactions, commonly associated with E3 ubiquitin ligases, while the SPRY domain facilitates specific binding to target proteins or membranes. BSPRY is evolutionarily conserved and predominantly expressed in tissues such as the kidney, lung, and reproductive organs, suggesting tissue-specific regulatory roles.
Functionally, BSPRY is implicated in modulating intracellular signaling pathways, including membrane receptor trafficking, ubiquitination, and immune responses. Studies highlight its interaction with TRIM family proteins, influencing NF-κB and MAPK pathways, which are critical for inflammation and apoptosis. Additionally, BSPRY may regulate ion channel activity, particularly in renal tubular cells, linking it to electrolyte homeostasis and kidney function.
Dysregulation of BSPRY is associated with pathological conditions. For example, reduced BSPRY expression correlates with renal fibrosis and chronic kidney disease, while aberrant activity has been observed in certain cancers, such as prostate and hepatocellular carcinoma. Its role in autophagy and cell survival further underscores potential therapeutic relevance in degenerative or hyperproliferative diseases.
Despite progress, the precise molecular mechanisms of BSPRY remain incompletely understood. Current research focuses on elucidating its interactome, post-translational modifications, and tissue-specific functions using knockout models and proteomic approaches. These efforts aim to clarify its contributions to human health and disease.
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