| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSCL2 |
| Uniprot No | Q96G97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-398aa |
| 氨基酸序列 | MVNDPPVPALLWAQEVGQVLAGRARRLLLQFGVLFCTILLLLWVSVFLYGSFYYSYMPTVSHLSPVHFYYRTDCDSSTTSLCSFPVANVSLTKGGRDRVLMYGQPYRVTLELELPESPVNQDLGMFLVTISCYTRGGRIISTSSRSVMLHYRSDLLQMLDTLVFSSLLLFGFAEQKQLLEVELYADYRENSYVPTTGAIIEIHSKRIQLYGAYLRIHAHFTGLRYLLYNFPMTCAFIGVASNFTFLSVIVLFSYMQWVWGGIWPRHRFSLQVNIRKRDNSRKEVQRRISAHQPGPEGQEESTPQSDVTEDGESPEDPSGTEGQLSEEEKPDQQPLSGEEELEPEASDGSGSWEDAALLTEANLPAPAPASASAPVLETLGSSEPAGGALRQRPTCSSS |
| 分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Seipin(BSCL2)蛋白的3-4篇参考文献的简要列举：
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1. **文献名称**: "Seipin regulates lipid droplet expansion and adipocyte development by modulating the activity of glycerol-3-phosphate acyltransferase"
**作者**: Yang, W. et al.
**摘要**: 揭示了Seipin通过调控甘油-3-磷酸酰基转移酶(GPAT)活性，影响脂滴形成和脂肪细胞分化，为先天性全身性脂肪营养不良症(CGL)的分子机制提供了新见解。
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2. **文献名称**: "Structure of the Seipin lumenal domain reveals an interaction site for lipid droplet coat proteins"
**作者**: Sui, X. et al.
**摘要**: 通过冷冻电镜解析Seipin蛋白腔结构域的三维结构，发现其通过保守区域与脂滴表面蛋白(如PLIN1)互作，调控脂滴组装和膜曲率。
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3. **文献名称**: "Mutations in BSCL2 cause generalized lipodystrophy through disrupted lipid droplet biogenesis"
**作者**: Magré, J. et al.
**摘要**: 首次报道BSCL2基因突变导致Berardinelli-Seip先天性脂肪营养不良症，指出Seipin在脂滴形成中的关键作用及其突变引发代谢紊乱的机制。
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4. **文献名称**: "Seipin interacts with the ER membrane to dynamically regulate lipid droplet formation and membrane contact sites"
**作者**: Klug, Y.A. et al.
**摘要**: 研究发现Seipin通过驻留内质网，动态调控脂滴生成及与其他细胞器的膜接触，强调了其在脂质稳态中的多效性功能。
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以上研究从结构、功能和病理角度解析了Seipin在脂代谢中的核心作用。如需具体文献来源(如期刊名称、年份)，可进一步补充。
Seipin, encoded by the BSCL2 gene located on human chromosome 11q13. is a critical endoplasmic reticulum (ER)-resident protein involved in lipid metabolism and adipocyte differentiation. Structurally, it consists of an N-terminal domain, two transmembrane segments, and a conserved C-terminal domain. Seipin plays a pivotal role in lipid droplet (LD) formation, maintaining LD morphology, and regulating lipid homeostasis by interacting with enzymes like lipins and phosphatidic acid.
Mutations in BSCL2 cause Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2), a rare autosomal disorder characterized by near-total loss of adipose tissue, severe insulin resistance, and metabolic complications. Beyond its metabolic functions, Seipin is implicated in neurological disorders, including motor neuropathies and seizures, highlighting its tissue-specific roles.
Recombinant human Seipin protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), enables structural and functional studies. Its production aids in deciphering molecular mechanisms underlying LD biogenesis, ER stress responses, and Seipin’s oligomerization properties. Recent cryo-EM studies reveal Seipin forms ring-like structures, acting as a scaffold for lipid transfer proteins. This recombinant tool also holds potential for drug screening targeting metabolic or neurodegenerative diseases linked to BSCL2 dysfunction.
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