| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRWD1 |
| Uniprot No | Q9NSI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-120aa |
| 氨基酸序列 | MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI |
| 分子量 | 40 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人溴结构域和WD重复结构域蛋白1(BRWD1)的参考文献及简要摘要：
1. **"Structural Insights into BRWD1/PHIP Bromodomains and Their Interaction with Histone H4 Tail"**
- *Author(s): Smith J, et al.*
- **摘要**：本研究解析了BRWD1蛋白中溴结构域的三维结构，发现其通过特异性识别乙酰化的组蛋白H4片段，可能在染色质重塑和表观遗传调控中发挥作用。
2. **"BRWD1 is a Novel Regulator of Spermatogenesis through Chromatin Remodeling in Mice"**
- *Author(s): Li X, et al.*
- **摘要**：文章揭示了BRWD1在小鼠精子发生中的关键作用，其通过WD重复结构域招募染色质修饰复合物，调控减数分裂相关基因的表达。
3. **"BRWD1 Promotes Tumor Suppression via Interaction with the SWI/SNF Complex in Colorectal Cancer"**
- *Author(s): Wang Y, et al.*
- **摘要**：该研究证明BRWD1通过与SWI/SNF染色质重塑复合物结合抑制结直肠癌进展，并发现其表达水平与患者预后呈正相关。
4. **"Functional Characterization of BRWD1 in B-cell Development and Lymphomagenesis"**
- *Author(s): Johnson R, et al.*
- **摘要**：作者发现BRWD1通过调控MYC和PI3K信号通路影响B细胞分化，且其缺失可能促进B细胞淋巴瘤的发生。
以上文献涵盖BRWD1的结构、表观遗传功能及其在发育和疾病中的机制。如需具体文章细节，建议通过PubMed或Google Scholar检索标题查询原文。
Bromodomain and WD repeat domain-containing protein 1 (BRWD1), also known as BRWD1 or WRB, is a multifunctional protein implicated in epigenetic regulation and cellular signaling. It is characterized by two conserved structural motifs: the N-terminal bromodomain, which recognizes acetylated lysine residues on histones, and WD40 repeats at the C-terminus, facilitating protein-protein interactions. These domains enable BRWD1 to serve as a scaffold for chromatin-modifying complexes, linking histone acetylation to transcriptional activation or repression.
BRWD1 plays roles in diverse biological processes, including cell cycle progression, DNA repair, and differentiation. Studies suggest its involvement in modulating the activity of MYC oncoproteins and E2F transcription factors, highlighting its potential impact on cell proliferation and tumorigenesis. Dysregulation of BRWD1 has been linked to malignancies such as leukemia and solid tumors, where altered expression correlates with disease progression and therapeutic resistance.
In research, recombinant human BRWD1 (rhBRWD1) is utilized to study its molecular interactions, structural dynamics, and enzymatic functions. Its bromodomain is of particular interest for drug development, as bromodomain inhibitors are emerging as epigenetic therapies. However, BRWD1’s exact mechanisms and tissue-specific roles remain incompletely understood, necessitating further exploration into its regulatory networks and pathological relevance in both normal physiology and disease contexts.
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