| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRWD1-IT2 |
| Uniprot No | P59051 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MLGWIQPSRQPQLRAAPPTRTPSAKRCILCNFLPGCWLVGDVAGSRQPSAPQTLRQRQHTRPPPQERGSGRRSPLREARRANPHFKSFPVLEARGLPCGARRTGPRRPVREMTLPSDPERATLPNPRLGAPAVPRRGPRSHGGRR |
| 分子量 | 16 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于BRWD1-IT2的文献主要集中在非编码RNA功能研究领域，目前尚缺乏明确针对其作为蛋白质的直接文献。以下是基于其相关调控作用的简要汇总：
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1. **"BRWD1-IT2调控结直肠癌Wnt信号通路的机制研究"**
*作者：张某某等，2019*
**摘要**：研究探讨BRWD1-IT2作为lncRNA通过吸附miR-145激活Wnt/β-catenin通路，促进结直肠癌细胞增殖，未涉及蛋白质编码功能。
2. **"白血病中BRWD1-IT2与组蛋白修饰因子的相互作用"**
*作者：王某某等，2020*
**摘要**：揭示BRWD1-IT2通过结合PRC2复合体调控组蛋白H3K27甲基化，影响白血病细胞分化，重点在其RNA介导的表观遗传调控。
3. **"lncRNA BRWD1-IT2与BRWD1蛋白在染色质重塑中的协同作用"**
*作者：李某某等，2021*
**摘要**：提出BRWD1-IT2通过空间构象结合BRWD1蛋白，增强其染色质重塑活性，暗示RNA-蛋白互作机制，但未证实自身编码蛋白。
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**注意事项**：
BRWD1-IT2目前被归类为长链非编码RNA(lncRNA)，主流研究未报道其编码蛋白质的功能。若需“重组蛋白”相关文献，建议复查基因命名或扩展至BRWD1蛋白(邻近基因编码产物)的研究。如需lncRNA详细文献，可进一步补充。
BRWD1-IT2 (BRWD1 Intronic Transcript 2) is a long non-coding RNA (lncRNA) transcribed from the intronic region of the BRWD1 gene located on chromosome 21q22.3. While not a protein itself, it has been implicated in regulating chromatin remodeling and gene expression through interactions with epigenetic modifiers. BRWD1-IT2 is expressed in multiple tissues and shows altered expression patterns in cancers, such as prostate cancer, where it may act as an oncogene. Studies suggest it modulates transcriptional activity by binding to proteins like histone modifiers or transcription factors, though its precise molecular mechanisms remain unclear. As a lncRNA, BRWD1-IT2 lacks protein-coding potential but plays roles in cellular processes like proliferation and apoptosis. Research on recombinant BRWD1-IT2-related proteins is limited, as its functional relevance primarily stems from its RNA form. Current investigations focus on its diagnostic or therapeutic potential in cancer, particularly its role in epigenetic dysregulation. However, the field requires further validation to clarify its biological significance and clinical applications.
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