| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRUNOL6 |
| Uniprot No | Q96J87 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481aa |
| 氨基酸序列 | MTAAPGGSAQPAGPGPRLGFSTADSGVGMSGLNPGPAVPMKDHDAIKLFVGQIPRGLDEQDLKPLFEEFGRIYELTVLKDRLTGLHKGCAFLTYCARDSALKAQSALHEQKTLPGMNRPIQVKPAASEGRGEDRKLFVGMLGKQQGEEDVRRLFQPFGHIEECTVLRSPDGTSKGCAFVKFGSQGEAQAAIRGLHGSRTMAGASSSLVVKLADTDRERALRRMQQMAGHLGAFHPAPLPLGACGAYTTAILQHQAALLAAAQGPGLGPVAAVAAQMQHVAAFSLVAAPLLPAAAANSPPGSGPGTLPGLPAPIGVNGFGPLTPQTNGQPGSDTLYNNGLSPYPAQSPGVADPLQQAYAGMHHYAAAYPSAYAPVSTAFPQQPSALPQQQREGPEGCNLFIYHLPQEFGDAELIQTFLPFGAVVSAKVFVDRATNQSKCFGFVSFDNPTSAQTAIQAMNGFQIGMKRLKAQLKRPKDANRPY |
| 分子量 | 76.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRUNOL6(CELF6)蛋白的3篇参考文献，涵盖功能、疾病关联及结构研究领域：
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1. **文献名称**：*CELF6 regulates RNA splicing and neurotransmission in mouse neuronal development*
**作者**：Smith, J.P. et al.
**摘要**：本研究通过重组表达人BRUNOL6蛋白，证实其作为RNA结合蛋白在神经元分化中的作用。实验表明，BRUNOL6调控突触相关mRNA(如Synaptotagmin)的剪接，并影响神经递质释放。研究为神经发育障碍机制提供了新见解。
2. **文献名称**：*BRUNOL6 interacts with tumor suppressors in colorectal cancer via recombinant protein assays*
**作者**：Wang, L. et al.
**摘要**：作者利用重组BRUNOL6蛋白进行体外结合实验，揭示其与肿瘤抑制蛋白APC的相互作用。结果表明，BRUNOL6过表达可能通过破坏RNA稳定性促进结直肠癌进展，为癌症靶向治疗提供潜在方向。
3. **文献名称**：*Structural insights into the RNA recognition motifs of BRUNOL6/CELF6*
**作者**：Garcia, R. et al.
**摘要**：通过表达纯化重组BRUNOL6蛋白并进行X射线晶体学分析，本研究解析了其RNA结合域的结构。结构数据揭示特异性核苷酸识别模式，解释了BRUNOL6在mRNA选择性剪接中的分子机制。
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以上文献均为虚构示例，实际研究中建议通过PubMed或Google Scholar以“BRUNOL6”、“CELF6”、“recombinant”为关键词检索真实文献。
BRUNOL6. also known as CELF6 (CUGBP Elav-like family member 6), is a RNA-binding protein belonging to the CELF/BRUNO family, characterized by conserved domains involved in RNA recognition and regulation. It plays a critical role in post-transcriptional gene regulation, including mRNA splicing, stability, and translation. Structurally, it contains RNA-binding motifs that enable interaction with specific sequences, often in the 3' untranslated regions (UTRs) of target mRNAs. BRUNOL6 is highly expressed in neuronal tissues, suggesting a functional significance in neurodevelopment, synaptic plasticity, and nervous system maintenance. Studies link its dysregulation to neurological disorders, such as autism spectrum disorders and intellectual disabilities, as well as certain cancers, where it may influence tumor progression via mRNA metabolism.
Recombinant BRUNOL6 protein is typically produced using bacterial or mammalian expression systems, allowing researchers to study its biochemical properties, RNA-binding specificity, and interactions with regulatory networks. Its recombinant form is vital for in vitro assays, structural studies, and screening potential therapeutic compounds targeting RNA-protein interactions. Additionally, it serves as a tool to dissect mechanisms underlying RNA processing defects in disease models. Despite progress, the full spectrum of BRUNOL6’s biological roles and molecular targets remains under investigation, highlighting its potential as both a biomarker and a therapeutic target in neuropathologies and oncology.
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