| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRUNOL5 |
| Uniprot No | Q8N6W0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MARLTESEARRQQQQLLQPRPSPVGSSGPEPPGGQPDGMKDLDAIKLFVGQIPRHLDEKDLKPLLEQFGRIYELTVLKDPYTGMHKGCAFLTYCARDSAIKAQTALHEQKTLPGMARPIQVKPADSESRGGRDRKLFVGMLNKQQSEEDVLRLFQPFGVIDECTVLRGPDGSSKGCAFVKFSSHTEAQAAIHALHGSQTMPGASSSLVVKFADTDKERTLRRMQQMVGQLGILTPSLTLPFSPYSAYAQALMQQQTTVLSTSGSYLSPGVAFSPCHIQQIGAVSLNGLPATPIAPASGLHSPPLLGTTAVPGLVAPITNGFAGVVPFSRWAPCPGNRLCQWPCALPSSEPDCGRDTASCLLRSPAVHSHVPHRGHHAHRAQRPPAAAPPAAAAARRSLETRS |
| 分子量 | 69.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRUNOL5蛋白的参考文献示例(请注意，BRUNOL5相关研究有限，以下内容为模拟文献，供参考格式)：
1. **文献名称**: "Expression and RNA-binding properties of recombinant human BRUNOL5 in neuronal development"
**作者**: Lee, S. et al.
**摘要**: 本研究在大肠杆菌中表达了重组人BRUNOL5蛋白，验证其通过CELF结构域与特定mRNA结合的能力，并发现其在小鼠神经元分化中对靶基因(如MAP2)剪接调控的潜在作用。
2. **文献名称**: "Structural insights into BRUNOL5's role in mRNA stability regulation"
**作者**: Zhang, Y. et al.
**摘要**: 通过X射线晶体学解析BRUNOL5蛋白的RNA结合域结构，揭示了其与AU-rich元件结合的分子机制，并证实重组蛋白在体外可抑制炎症因子(如TNF-α)mRNA的降解。
3. **文献名称**: "BRUNOL5 knockdown and overexpression models in cancer cell proliferation"
**作者**: Garcia, R. et al.
**摘要**: 利用重组BRUNOL5蛋白验证其在胶质母细胞瘤中的作用，发现其通过调控CD44可变剪接抑制肿瘤侵袭，提示其作为治疗靶点的潜力。
4. **文献名称**: "High-yield purification of BRUNOL5 for functional genomics studies"
**作者**: Müller, P. et al.
**摘要**: 开发了一种基于HEK293细胞的重组BRUNOL5蛋白表达系统，优化纯化流程后获得高活性蛋白，用于高通量筛选其RNA靶标及药物相互作用研究。
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**注意**：上述文献为模拟示例，实际研究中BRUNOL5的研究可能较少(部分功能可能与CELF家族其他成员混淆)。建议通过PubMed或Google Scholar以“BRUNOL5”或“CELF5”结合“recombinant protein”等关键词检索最新文献。
BRUNOL5 (also known as CELF5) is a member of the CUG-BP and ETR-3-like factor (CELF) family of RNA-binding proteins, which play critical roles in post-transcriptional gene regulation. Structurally, it contains conserved RNA recognition motifs (RRMs) that enable binding to specific RNA sequences, influencing pre-mRNA splicing, mRNA stability, and translation. Initially identified through homology with Bruno protein in *Drosophila*, BRUNOL5 is predominantly expressed in neuronal tissues and testis, suggesting specialized functions in neurodevelopment and gametogenesis.
Research highlights its involvement in neural differentiation and synaptic plasticity by regulating mRNA targets related to ion channels and synaptic proteins. Dysregulation of BRUNOL5 has been implicated in neurological disorders, including autism spectrum disorders and intellectual disabilities. Additionally, its aberrant expression is observed in certain cancers, where it may modulate oncogenic or tumor-suppressive pathways through RNA metabolism. Recombinant BRUNOL5 protein is typically produced in *E. coli* or mammalian expression systems for functional studies, enabling insights into its molecular interactions and regulatory mechanisms. Current studies focus on elucidating its target RNAs and signaling networks, positioning BRUNOL5 as a potential therapeutic target or biomarker in neurological and oncological contexts.
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