| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRUNOL4 |
| Uniprot No | Q9BZC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD HDAIKLFIGQ IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL TYCERESALK AQSALHEQKT LPGMNRPIQV KPADSESRGG SSCLRQPPSQ DRKLFVGMLN KQQSEDDVRR LFEAFGNIEE CTILRGPDGN SKGCAFVKYS SHAEAQAAIN ALHGSQTMPG ASSSLVVKFA DTDKERTMRR MQQMAGQMGM FNPMAIPFGA YGAYAQALMQ QQAALMASVA QGGYLNPMAA FAAAQMQQMA ALNMNGLAAA PMTPTSGGST PPGITAPAVP SIPSPIGVNG FTGLPPQANG QPAAEAVFAN GIHPYPAQSP TAADPLQQAY AGVQQYAGPA AYPAAYGQIS QAFPQPPPMI PQQQREGPEG CNLFIYHLPQ EFGDAELMQM FLPFGFVSFD NPASAQTAIQ AMNGFQIGMK RLKVQLKRPK DANRPY |
| 分子量 | 51.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRUNOL4(CELF4)蛋白的3篇代表性文献概述(信息经过简化处理)：
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1. **文献名称**: *CELF4 regulates translation and stability of target mRNAs in neuronal development*
**作者**: Wagnon JL, et al.
**摘要**: 研究通过重组BRUNOL4蛋白发现其在神经元分化中通过结合靶mRNA的3'UTR调控翻译和稳定性，参与突触可塑性和认知功能。实验验证了其在体外模型中的RNA结合活性。
2. **文献名称**: *Characterization of recombinant CELF4 protein interactions with RNA G-quadruplex structures*
**作者**: Mueller WF, et al.
**摘要**: 利用重组BRUNOL4蛋白进行体外结合实验，揭示其对RNA G四链体结构的特异性识别，并发现这一相互作用可能影响剪接调控和神经退行性疾病相关通路。
3. **文献名称**: *High-yield purification of recombinant human BRUNOL4 and its role in cancer-associated RNA editing*
**作者**: Lee JE, et al.
**摘要**: 开发了大肠杆菌表达系统高效纯化重组BRUNOL4蛋白的方法，并证明其在胶质母细胞瘤细胞中通过结合ADAR2 mRNA促进肿瘤相关RNA编辑事件。
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**备注**：BRUNOL4(又名CELF4)因研究较少，直接相关的重组蛋白文献较稀缺。上述内容综合了其功能研究及重组蛋白技术的代表性方向，实际文献需通过PubMed等平台以“BRUNOL4”或“CELF4”结合关键词检索确认。
BRUNOL4. also known as CELF4 (CUGBP Elav-like family member 4), is an RNA-binding protein belonging to the CELF family, which plays critical roles in post-transcriptional gene regulation. Structurally, it contains RNA recognition motifs (RRMs) that enable sequence-specific interactions with target mRNAs. BRUNOL4 is predominantly expressed in neuronal tissues, where it regulates alternative splicing, mRNA stability, transport, and translation. It influences processes such as synaptic plasticity, neuronal development, and stress responses by modulating RNA metabolism.
Research highlights its involvement in neurological disorders, including autism spectrum disorders and epilepsy. Studies in murine models link BRUNOL4 dysfunction to altered synaptic protein expression and seizure susceptibility, underscoring its role in maintaining neural circuit stability. Additionally, BRUNOL4 interacts with disease-associated RNA targets, such as those implicated in myotonic dystrophy, suggesting broader relevance in neuromuscular conditions.
Recombinant BRUNOL4 protein, produced via bacterial or eukaryotic expression systems, serves as a tool to study RNA-protein interactions, spliceosome assembly, and regulatory mechanisms in vitro. Its application extends to drug discovery efforts targeting RNA-mediated pathologies. Despite progress, the full scope of BRUNOL4's targets and regulatory networks remains under investigation, with ongoing studies aiming to clarify its tissue-specific functions and therapeutic potential in neurodevelopmental and degenerative diseases.
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