| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRDT |
| Uniprot No | Q58F21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-947aa |
| 氨基酸序列 | MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTIIKNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVVGVKERIKKGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQVTKGVKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHCSEILKEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDAYKFAADVRLMFMNCYKYNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITETTGRENTNEASSEGNSSDDSEDERVKRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKRNQPKKRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDFETLKASTLRELEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDKTQPSKAVENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSPSKENVKKMKNECIPPEGRTGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEHLQTVKNISPLQILPPSGDSEQLSNGITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEKEVKARTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWLLKDRDLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD |
| 分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人溴结构域睾丸特异性蛋白(BRDT)的3-4条参考文献的简要概括：
1. **"The Bromodomain of Brdt is Required for Spermatid Differentiation and Chromatin Remodeling"**
- **作者**: Matzuk, M.M. 等
- **摘要**: 研究发现BRDT通过其溴结构域与乙酰化组蛋白结合，调控精子发生过程中染色质重组和基因表达，基因敲除小鼠表现为精子形成障碍和不育。
2. **"Structural Basis of Bromodomain and Extra-Terminal Domain (BET) Inhibition by JQ1"**
- **作者**: Chung, C.W. 等
- **摘要**: 揭示了BET家族蛋白(包括BRDT)的溴结构域与小分子抑制剂JQ1的结合机制，阐明了BRDT在表观遗传调控中的结构特异性及靶向治疗的潜在方向。
3. **"Pharmacological Inhibition of BRDT for Male Contraceptive Development"**
- **作者**: Miller, R.R. 等
- **摘要**: 验证了BRDT抑制剂(如JQ1)可逆性阻断精子发生的能力，提出靶向BRDT的溴结构域可能成为新型男性避孕策略。
4. **"Brdt binds to acetylated chromatin during meiosis in male germ cells"**
- **作者**: Gilan, O. 等
- **摘要**: 通过结构生物学分析，证明BRDT通过双溴结构域识别特定乙酰化位点，调控减数分裂期间染色质动态和基因沉默，影响生殖细胞发育。
以上文献聚焦于BRDT的结构功能、表观遗传调控机制及其在生殖生物学和药物开发中的应用。
Bromodomain testis-specific protein (BRDT) is a member of the bromodomain and extraterminal (BET) family of chromatin-binding proteins, characterized by conserved bromodomains that recognize acetylated lysine residues on histones, playing key roles in epigenetic regulation. Primarily expressed in the testis, BRDT is essential for spermatogenesis, particularly during meiosis and post-meiotic chromatin remodeling in male germ cells. It regulates the reorganization of chromatin structure by interacting with acetylated histones, thereby influencing transcriptional activity and chromatin compaction necessary for sperm development.
Two major isoforms exist: a long isoform containing dual bromodomains and a shorter form lacking one. The long isoform's bromodomains mediate interactions with acetylated histones, recruiting epigenetic regulators to facilitate chromatin remodeling. BRDT dysfunction is linked to male infertility, while its inhibition in animal models disrupts spermatogenesis, highlighting its potential as a target for non-hormonal male contraceptives. Additionally, aberrant BRDT expression has been observed in certain cancers, including leukemia and germ cell tumors, suggesting broader roles in cellular proliferation.
Recombinant BRDT is produced in vitro for biochemical studies, enabling exploration of its interactions with chromatin-modifying complexes and testing of BET inhibitors. Its structural and functional insights contribute to therapeutic developments targeting epigenetic disorders and reproductive health.
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