| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOP1 |
| Uniprot No | P97452 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-746aa |
| 氨基酸序列 | MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNTVGNVPLEWYDDFPHVGYDLDGRRIYKPLRTRDELDQFLDKMDDPDYWRTVQDPMTGRDLRLTDEQVALVRRLQSGQFGDVGFNPYEPAVDFFSGDVMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIQPRRPRDPTPSFYDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLLSEEERLAWEQQEPGERKLSFLPRKFPSLRAVPAYGRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPTCQALVYRGHSDLVRCLSVSPGGQWLVSGSDDGSLRLWEVATARCVRTVPVGGVVKSVAWNPSPAVCLVAAAVEDSVLLLNPALGDRLVAGSTDQLLSAFVPPEEPPLQPARWLEASEEERQVGLRLRICHGKPVTQVTWHGRGDYLAVVLATQGHTQVLIHQLSRRRSQSPFRRSHGQVQRVAFHPARPFLLVASQRSVRLYHLLRQELTKKLMPNCKWVSSLAVHPAGDNVICGSYDSKLVWFDLDLSTKPYRMLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVKVLKGHVLTRDLGVLDVIFHPTQPWVFSSGADGTVRLFT |
| 分子量 | 110 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BOP1蛋白的3篇代表性文献摘要总结：
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1. **文献名称**："BOP1 links ribosomal RNA processing to p53-mediated cell cycle arrest"
**作者**：Pestov DG, et al.
**摘要**：研究发现BOP1在核糖体RNA(rRNA)加工中起关键作用，并通过重组人BOP1蛋白表达证实，其功能缺陷会激活p53通路，导致细胞周期停滞，提示BOP1在癌症调控中的潜在角色。
2. **文献名称**："Recombinant expression and functional analysis of human BOP1 in ribosome biogenesis"
**作者**：Strezoska Ž, et al.
**摘要**：利用大肠杆菌系统成功表达重组人BOP1蛋白，证明其与PES1和WDR12形成复合物，参与28S rRNA的成熟，为研究核糖体合成缺陷相关疾病提供工具。
3. **文献名称**："BOP1 is required for ribosome biogenesis and its depletion triggers autophagy"
**作者**：Grimm T, et al.
**摘要**：通过siRNA敲低和重组BOP1互补实验发现，BOP1缺失导致核仁应激，进而激活自噬通路，揭示了BOP1在维持细胞稳态中的双重功能。
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注：以上文献为模拟示例，实际引用请核对具体论文数据库(如PubMed)或学术引擎。
**Background of Recombinant Human BOP1 Protein**
BOP1 (Block of Proliferation 1) is a conserved WD40-repeat-containing protein involved in ribosome biogenesis and cell cycle regulation. It interacts with PES1 and WDR12 to form the PeBoW complex, which facilitates rRNA processing during the maturation of the 60S ribosomal subunit. BOP1 is critical for proper nucleolar function, and its depletion disrupts ribosome assembly, leading to cell cycle arrest and p53-dependent growth inhibition. Studies link BOP1 to proliferation control, as its overexpression induces genomic instability, while its deficiency triggers cell senescence or apoptosis.
Recombinant human BOP1 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies of its role in ribosome synthesis, cancer biology, and cellular stress responses. It serves as a tool to explore molecular mechanisms underlying diseases linked to ribosomopathies or uncontrolled proliferation, such as cancers. Research using recombinant BOP1 also aids in identifying potential therapeutic targets by dissecting its interactions within nucleolar pathways and DNA damage signaling networks. Its structural and functional conservation across species underscores its broad relevance in basic and translational biology.
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