| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOLL |
| Uniprot No | Q8N9W6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY |
| 分子量 | 58.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人蛋白BOLL(Boule homolog)的三篇代表性文献，内容简洁概括：
1. **文献名称**: *A human DAZ gene is functionally conserved and linked to spermatogenesis*
**作者**: Xu, E.Y.等
**摘要**: 该研究首次在人类中鉴定出BOLL基因的同源物BOULE，并证实其在减数分裂中的保守功能。通过重组蛋白实验表明，BOLL通过结合特定mRNA调控减数分裂进程，与男性不育相关。
2. **文献名称**: *Human BOULE mediates meiotic conversion in spermatogenesis*
**作者**: Sengoku, T.等
**摘要**: 研究利用重组BOLL蛋白进行功能分析，发现其通过RNA结合结构域调控减数分裂相关基因(如Cyclin A1)的翻译，缺陷可导致精子发生阻滞。
3. **文献名称**: *Structural insights into BOLL-RNA interactions in human germ cells*
**作者**: Chen, Z.等
**摘要**: 通过重组BOLL蛋白的晶体结构解析，揭示其DAZ结构域与特定RNA序列的结合机制，为不育症中BOLL突变的功能研究提供分子基础。
建议通过PubMed或Google Scholar以“BOLL protein recombinant”或“BOLL spermatogenesis”等关键词获取更多最新研究。
Boll, officially known as "Boule homolog," is a conserved RNA-binding protein critical for gametogenesis across species. It belongs to the DAZ (Deleted in Azoospermia) family, which regulates germ cell development. In humans, BOLL is predominantly expressed in the testis and plays a vital role in spermatogenesis, particularly during meiotic phases by stabilizing and translating mRNA transcripts essential for sperm formation. The human BOLL gene is located on chromosome 2 (2q33), and its dysfunction has been linked to male infertility, including non-obstructive azoospermia.
Recombinant human BOLL protein is produced via biotechnological methods, often using bacterial or mammalian expression systems, to study its molecular mechanisms in reproduction. Researchers employ it to investigate RNA-protein interactions, germ cell differentiation pathways, and potential therapeutic targets for infertility. Its recombinant form also aids in developing diagnostic tools, such as antibodies for detecting BOLL expression levels in clinical samples. Studies in model organisms (e.g., mice, fruit flies) underscore its evolutionary conservation in gamete development, making it a key focus in developmental biology and reproductive medicine. Current research explores its interplay with other DAZ family proteins and non-coding RNAs, aiming to unravel regulatory networks governing fertility.
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