| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOLA2 |
| Uniprot No | Q9H3K6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-124aa |
| 氨基酸序列 | MASAKSLDRWKARLLEGGSTALTYALVRAEVSFPAEVAPVRQQGSVAGARAGVVSLLGCRSSWTAAMELSAEYLREKLQRDLEAEHVEVEDTTLNRCSCSFRVLVVSAKFEGKPLLQRHRFCTE |
| 分子量 | 13.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BOLA样蛋白2(BOLA2)的模拟参考文献示例(注意：以下为假设文献，建议通过学术数据库验证实际存在的文献)：
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1. **标题**：*Structural insights into human BOLA2 and its interaction with glutaredoxin in iron-sulfur cluster assembly*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析BOLA2的构象，揭示其与谷氧还蛋白(Grx3)的结合界面，表明BOLA2可能在铁硫簇的转移与代谢中起桥梁作用。
2. **标题**：*Recombinant human BOLA2 regulates mitochondrial iron homeostasis under oxidative stress*
**作者**：Lee S, et al.
**摘要**：研究发现重组BOLA2在线粒体铁稳态中起关键作用，过表达可减轻氧化应激导致的细胞损伤，提示其作为抗氧化治疗靶点的潜力。
3. **标题**：*BOLA2 deficiency disrupts erythroid development via impaired heme biosynthesis*
**作者**：Zhang Y, et al.
**摘要**：通过基因编辑技术证实，BOLA2缺失导致血红素合成通路关键酶活性下降，影响红细胞生成，提示其与铁代谢疾病的关联。
4. **标题**：*High-throughput expression and functional screening of recombinant BOLA2 in human cell lines*
**作者**：Garcia R, et al.
**摘要**：开发了一种高效重组BOLA2表达纯化系统，并验证其通过与伴侣蛋白BolA同源物协同调控细胞增殖的功能。
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**注意**：以上文献为模拟示例，具体内容需以真实文献为准。建议使用 **PubMed**、**Google Scholar** 或 **Web of Science**，以关键词“BOLA2”、“iron-sulfur cluster”、“recombinant BOLA2”等检索最新研究。
**Background of Recombinant Human BOLA-Like Protein 2 (BOLA2)**
BOLA-like protein 2 (BOLA2) is a member of the evolutionarily conserved BOLA protein family, implicated in cellular iron homeostasis and iron-sulfur (Fe-S) cluster metabolism. In humans, BOLA2 is encoded by the *BOLA2* gene, located within the 16p11.2 chromosomal region, a locus associated with neurodevelopmental disorders. BOLA2 shares structural homology with bacterial BolA proteins, which regulate Fe-S cluster assembly and stress response pathways.
Functionally, BOLA2 interacts with glutaredoxins (e.g., GLRX3), forming complexes that facilitate Fe-S cluster transfer and redox regulation. This interaction is critical for maintaining cellular iron balance and mitigating oxidative stress. Studies suggest BOLA2’s role in mitochondrial Fe-S protein maturation and cytosolic iron regulation, linking it to metabolic and oxidative stress-related processes.
Recombinant BOLA2 is produced via heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling biochemical and structural studies. Its recombinant form retains the ability to bind iron and participate in Fe-S cluster biosynthesis, making it a tool for exploring metal ion trafficking and redox signaling. Additionally, BOLA2’s genetic proximity to *SEPHS2*, a gene involved in selenium metabolism, hints at broader roles in nutrient sensing and homeostasis.
Dysregulation of BOLA2 has been tentatively linked to 16p11.2 deletion/duplication syndromes, though its exact contribution remains under investigation. Research continues to elucidate its molecular mechanisms and potential therapeutic relevance in iron-related disorders or neurodegenerative conditions.
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