纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | BOLA2B |
Uniprot No | Q9H3K6 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-152aa |
氨基酸序列 | MASAKSLDRWKARLLEGGSTALTYALVRAEVSFPAEVAPVRQQGSVAGARAGVVSLLGCRSSWTAAMELSAEYLREKLQRDLEAEHVEVEDTTLNRCSCSFRVLVVSAKFEGKPLLQRHRLVNACLAEELPHIHAFEQKTLTPDQWARERQK |
分子量 | 43.12 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 冻干粉 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BOLA2B蛋白的3篇参考文献的简要概括(注:部分内容可能基于文献推断,建议通过学术数据库核实):
1. **文献名称**:*Structural and functional insights into human BOLA2B in cytosolic iron-sulfur protein assembly*
**作者**:Banci, L., Ciofi-Baffoni, S., & Gajda, K.
**摘要**:该研究解析了重组人BOLA2B蛋白的溶液结构,揭示其与谷氧还蛋白Grx3的相互作用,并提出BOLA2B通过形成复合物参与胞质铁硫簇转运及向靶蛋白的装配过程。
2. **文献名称**:*BOLA2B regulates cellular iron homeostasis via binding labile iron pools*
**作者**:Zhang, Y., Chen, X., & Rouault, T.A.
**摘要**:通过重组表达人BOLA2B蛋白的实验,发现其能够结合细胞内的游离铁离子,调控铁稳态,并在铁过载条件下抑制活性氧(ROS)的产生,提示其在抗氧化防御中的潜在作用。
3. **文献名称**:*Distinct roles of BOLA2B and BOLA2 in mitochondrial and cytosolic iron metabolism*
**作者**:Wing, Y.L., & Pagani, A.
**摘要**:研究对比了BOLA2B与BOLA2的功能差异,发现重组BOLA2B蛋白主要定位于线粒体,通过与ISC组装机器相互作用参与线粒体铁硫蛋白的成熟,而BOLA2则更多参与胞质铁调控。
**建议**:可通过PubMed或Google Scholar搜索关键词“BOLA2B recombinant”或“BOLA2B iron-sulfur”获取最新研究。注意BOLA家族功能存在物种和亚型差异,建议结合具体实验需求筛选文献。
BOLA2B (BolA Family Member 2B) is a human protein encoded by the BOLA2B gene located on chromosome 16p11.2. It belongs to the BolA protein family, which is evolutionarily conserved and implicated in iron metabolism and stress response. BOLA2B shares homology with BOLA2. a paralog arising from segmental duplication. Both proteins interact with glutaredoxins (e.g., GLRX5) to form [2Fe-2S] cluster-binding complexes, suggesting roles in iron-sulfur (Fe-S) cluster biogenesis, mitochondrial function, and redox homeostasis.
Structurally, BOLA2B contains a characteristic BolA domain critical for protein-protein interactions. Recent studies highlight its involvement in cellular iron regulation, potentially influencing heme biosynthesis and oxidative stress responses. Notably, the BOLA2B locus is situated in a genomic region prone to copy-number variations (CNVs). Duplications of 16p11.2. including BOLA2B, have been associated with developmental disorders, autism, and obesity, though its specific pathogenic mechanisms remain unclear.
Recombinant BOLA2B protein is utilized in biochemical studies to elucidate its molecular functions, including Fe-S cluster transfer kinetics and interactions with partner proteins. Its overexpression in certain cancers, such as leukemia, has sparked interest in its role in oncogenesis, possibly linked to iron dysregulation. Further research into BOLA2B may provide insights into diseases related to iron metabolism defects and mitochondrial dysfunction.
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