| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMS1 |
| Uniprot No | Q14692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403aa |
| 氨基酸序列 | MGKVWKQQMYPQYATYYYPQYLQAKQSLVPAHPMAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLPPHTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELENMLKPFGQVISTRILRDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPPGVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRPWHREGEAGMTLTYDPTTAAIQNGFYPSPYSIATNRMITQTSITPYIASPVSAYQVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLGSTGTYMPATSAMQGAYLPQYAHMQTTAVPVEEASGQQQVAVETSNDHSPYTFQPNK |
| 分子量 | 70.07 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于已有知识整理的BMS1相关参考文献(建议通过学术数据库进一步验证准确性)：
1. **"BMS1 coordinates with RCL1 to mediate ribosomal RNA processing"**
- **作者**: Liu et al.
- **摘要**: 研究揭示了BMS1与RCL1蛋白协同作用，通过其GTP酶活性调控pre-rRNA的切割及成熟核糖体亚基的组装，表明BMS1在核糖体生物发生的早期阶段发挥关键作用。
2. **"Mutations in BMS1 cause cerebellar hypoplasia and neonatal lethality"**
- **作者**: Weaver et al.
- **摘要**: 通过小鼠模型发现BMS1基因突变可导致小脑发育异常及围产期死亡，暗示BMS1在神经发育中的必要性，并为相关遗传性疾病提供分子机制解释。
3. **"Structural insights into BMS1-mediated ribosome assembly"**
- **作者**: Zhang et al.
- **摘要**: 利用冷冻电镜解析BMS1蛋白的三维结构，阐明其与RNA解旋酶及核仁因子的互作界面，提出BMS1通过构象变化协调rRNA折叠的模型。
4. **"BMS1 expression correlates with tumor progression in hepatocellular carcinoma"**
- **作者**: Chen et al.
- **摘要**: 临床数据分析显示BMS1在肝癌组织中高表达，且与患者生存率负相关，机制研究提示其可能通过调控核糖体合成促进癌细胞增殖。
**注意**：以上文献信息为示例性质，具体内容需在PubMed、Google Scholar等平台以“BMS1”、“ribosome biogenesis”、“rRNA processing”等关键词检索核实。
BMS1 (BMS1 homolog, ribosome assembly protein) is a conserved GTP-binding protein critical for ribosomal RNA (rRNA) processing and ribosome biogenesis. It plays a central role in the maturation of the 40S ribosomal subunit by facilitating the cleavage of 18S rRNA from the 35S precursor transcript. Evolutionarily conserved from yeast to humans, BMS1 interacts with Rcl1 to form a functional complex that stabilizes early pre-ribosomal particles. Structurally, it contains a GTPase domain essential for its enzymatic activity and a C-terminal region involved in protein-protein interactions.
Dysregulation of BMS1 is linked to cellular growth abnormalities. Mutations or aberrant expression impair ribosome assembly, triggering nucleolar stress and p53-dependent cell cycle arrest. Such defects are associated with developmental disorders, including 5q- syndrome (a ribosomopathy characterized by bone marrow failure), and certain cancers where BMS1 overexpression correlates with tumor proliferation. Recent studies also suggest roles beyond ribosome synthesis, potentially influencing mRNA translation fidelity under stress. Its dual functionality as a GTPase and scaffolding protein makes BMS1 a key regulatory node connecting ribosome production to cellular homeostasis, highlighting its importance in both basic biology and disease mechanisms.
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