| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BLZF1 |
| Uniprot No | Q9H2G9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MTTKNLETKV TVTSSPIRGA GDGMETEEPP KSVEVTSGVQ SRKHHSLQSP WKKAVPSESP GVLQLGKMLT EKAMEVKAVR ILVPKAAITH DIPNKNTKVK SLGHHKGEFL GQSEGVIEPN KELSEVKNVL EKLKNSERRL LQDKEGLSNQ LRVQTEVNRE LKKLLVASVG DDLQYHFERL AREKNQLILE NEALGRNTAQ LSEQLERMSI QCDVWRSKFL ASRVMADELT NSRAALQRQN RDAHGAIQDL LSEREQFRQE MIATQKLLEE LLVSLQWGRE QTYSPSVQPH STAELALTNH KLAKAVNSHL LGNVGINNQK KIPSTVEFCS TPAEKMAETV LRILDPVTCK ESSPDNPFFE SSPTTLLATK KNIGRFHPYT RYENITFNCC NHCRGELIAL |
| 分子量 | 71.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人高尔基体蛋白45(BLZF1)的虚构参考文献，根据典型研究领域概括：
1. **文献名称**：*BLZF1 regulates Golgi structure and secretory pathway through interaction with GRASP55*
**作者**：Zhang, Y., Liu, X., & Wang, J. (2018)
**摘要**：研究发现BLZF1通过与GRASP55蛋白相互作用维持高尔基体堆叠结构完整性，调控细胞分泌途径。敲低BLZF1导致高尔基体碎片化并抑制蛋白质分泌。
2. **文献名称**：*BLZF1 suppresses tumor metastasis by modulating EGFR trafficking in hepatocellular carcinoma*
**作者**：Chen, L., et al. (2020)
**摘要**：首次揭示BLZF1在肝癌中作为肿瘤抑制因子，通过促进EGFR蛋白的高尔基体-细胞膜定向运输阻滞异常信号通路，抑制肿瘤转移。
3. **文献名称**：*Proteomic analysis identifies BLZF1 as a novel binding partner of Rab33B in vesicle tethering*
**作者**：Tanaka, K., et al. (2022)
**摘要**：基于蛋白质组学发现BLZF1与Rab33B直接互作，参与高尔基体来源囊栓的锚定过程，揭示其在小G蛋白介导的膜运输中的新机制。
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注：以上内容为基于BLZF1的已知生物学功能(如高尔基体结构调控、肿瘤关联、囊泡运输)生成的模拟文献，实际研究请通过PubMed/Google Scholar检索。
**Background of Human Golgi Matrix Protein 45 (BLZF1/Golgin-45)**
BLZF1 (B-lymphoma and zinc finger protein 1), also termed Golgin-45. is a member of the golgin family, a group of coiled-coil domain-containing proteins localized to the Golgi apparatus. It plays a role in maintaining Golgi structure and regulating vesicular transport, membrane fusion, and protein sorting. BLZF1 is characterized by its central coiled-coil domains and an N-terminal basic region, facilitating interactions with other Golgi components and membrane association.
Functionally, BLZF1 contributes to Golgi ribbon formation and stability, critical for proper secretory pathway function. It interacts with proteins like JADE1 (Jade family PHD finger 1), forming complexes that influence Golgi morphology and dynamics. Studies suggest its involvement in cellular processes such as apoptosis, autophagy, and cell differentiation, potentially mediated through signaling pathways like Hedgehog.
BLZF1 dysregulation has been linked to diseases, including cancers. Elevated expression is observed in hepatocellular carcinoma, colorectal cancer, and gliomas, where it may promote tumor progression via enhanced cell proliferation or resistance to apoptosis. Its role as a transcription regulator, possibly through zinc finger motifs, is under investigation.
Research continues to explore BLZF1’s molecular mechanisms and therapeutic potential, particularly in cancer biology and Golgi-related disorders.
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