| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BIRC4 |
| Uniprot No | P98170 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-497aa |
| 氨基酸序列 | MTFNSFEGSK TCVPADINKE EEFVEEFNRL KTFANFPSGS PVSASTLARA GFLYTGEGDT VRCFSCHAAV DRWQYGDSAV GRHRKVSPNC RFINGFYLEN SATQSTNSGI QNGQYKVENY LGSRDHFALD RPSETHADYL LRTGQVVDIS DTIYPRNPAM YSEEARLKSF QNWPDYAHLT PRELASAGLY YTGIGDQVQC FCCGGKLKNW EPCDRAWSEH RRHFPNCFFV LGRNLNIRSE SDAVSSDRNF PNSTNLPRNP SMADYEARIF TFGTWIYSVN KEQLARAGFY ALGEGDKVKC FHCGGGLTDW KPSEDPWEQH AKWYPGCKYL LEQKGQEYIN NIHLTHSLEE CLVRTTEKTP SLTRRIDDTI FQNPMVQEAI RMGFSFKDIK KIMEEKIQIS GSNYKSLEVL VADLVNAQKD SMQDESSQTS LQKEISTEEQ LRRLQEEKLC KICMDRNIAI VFVPCGHLVT CKQCAEAVDK CPMCYTVITF KQKIFMS |
| 分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人BIRC4(XIAP)蛋白的参考文献，简要概括内容如下：
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1. **Structural basis of caspase inhibition by XIAP**
- **作者**: Chai J. et al.
- **摘要**：通过X射线晶体学解析了XIAP BIR2结构域与caspase-3/7的相互作用机制，揭示其通过BIR结构域结合并抑制caspase活性的分子基础。研究使用重组XIAP蛋白进行结构分析。
2. **X-linked IAP protein inhibits apoptotic cell death by interfering with caspase-9 activation**
- **作者**: Deveraux Q.L. et al.
- **摘要**：首次报道XIAP通过直接结合并抑制caspase-9调控凋亡通路。重组XIAP蛋白实验表明其可阻断细胞色素c/Apaf-1介导的caspase激活，为凋亡抑制机制提供证据。
3. **Design of nonpeptidic small-molecule inhibitors of BIRC4/X-linked inhibitor of apoptosis**
- **作者**: Schimmer A.D. et al.
- **摘要**：基于重组BIRC4蛋白的高通量筛选，开发小分子拮抗剂(如AEG35156)，研究显示其可阻断XIAP的抗凋亡功能并增强肿瘤细胞对化疗的敏感性。
4. **Mechanism of XIAP-mediated inhibition of caspase-9**
- **作者**: Shiozaki E.N. et al.
- **摘要**：通过重组蛋白结合实验和突变分析，证明XIAP BIR3结构域直接结合caspase-9的活性位点，抑制其蛋白酶活性，进而调控线粒体凋亡通路。
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以上文献涉及重组BIRC4/XIAP蛋白的结构解析、功能机制及治疗应用，均发表于**Nature**、**Molecular Cell**等期刊。
Survivin, encoded by the *BIRC4* gene (Baculoviral IAP Repeat-Containing 4), is a member of the inhibitor of apoptosis protein (IAP) family. It plays dual roles in regulating apoptosis and cell cycle progression, primarily expressed during embryonic development but aberrantly overexpressed in many cancers. Structurally, Survivin contains a single baculoviral IAP repeat (BIR) domain critical for its anti-apoptotic function and a coiled-coil region for protein-protein interactions. It inhibits caspase activity by binding directly to caspases-3. -7. and -9. thereby blocking apoptotic signaling.
Notably, Survivin forms the chromosomal passenger complex (CPC) with Aurora B kinase and other partners, ensuring proper mitotic progression, spindle assembly, and cytokinesis. Its overexpression in tumors correlates with poor prognosis, chemotherapy resistance, and increased metastatic potential, making it a prominent therapeutic target. However, Survivin’s role extends beyond cancer; it maintains hematopoietic stem cell viability and modulates inflammatory responses.
Recombinant human Survivin (rhSurvivin) is produced via bacterial or eukaryotic expression systems, enabling studies on its molecular mechanisms and drug development. Research focuses on small-molecule inhibitors, antisense oligonucleotides, and immunotherapies targeting Survivin to restore apoptosis in cancer cells. Despite challenges in specificity and off-target effects, Survivin-based therapies hold promise due to its cancer-restricted expression. Ongoing studies also explore its involvement in non-cancer pathologies, including vascular and autoimmune disorders.
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