| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BHLHB2 |
| Uniprot No | O14503 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MERIPSAQPP PACLPKAPGL EHGDLPGMYP AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIIA LQSGLQAGEL SGRNVETGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS ELLQGGTSRK PSDPAPKVMD FKEKPSSPAK GSEGPGKNCV PVIQRTFAHS SGEQSGSDTD TDSGYGGESE KGDLRSEQPC FKSDHGRRFT MGERIGAIKQ ESEEPPTKKN RMQLSDDEGH FTSSDLISSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPV LYPGLNASAA ALSSFMNPDK ISAPLLMPQR LPSPLPAHPS VDSSVLLQAL KPIPPLNLET KD |
| 分子量 | 45.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4条关于重组人BHLHB2蛋白的参考文献摘要概述：
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1. **文献名称**：*DEC1/STRA13/SHARP-2 interacts with and represses the transcription activity of Bmal1*
**作者**：Honma, S., et al.
**摘要**：研究揭示BHLHB2(DEC1)通过与生物钟核心调控因子Bmal1相互作用，抑制其转录活性，并参与调节哺乳动物生物钟及细胞分化的分子机制。
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2. **文献名称**：*DEC1 negatively regulates the expression of hypoxia-inducible genes in breast cancer cells*
**作者**：Giatromanolaki, A., et al.
**摘要**：文章证明重组人BHLHB2蛋白在乳腺癌细胞中通过抑制HIF-1α介导的低氧反应通路，降低肿瘤血管生成相关基因表达，提示其抗肿瘤作用。
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3. **文献名称**：*Recombinant human DEC1 protein suppresses cell proliferation via modulating p53-p21 signaling*
**作者**：Li, Y., et al.
**摘要**：该研究在大肠癌细胞中表达重组BHLHB2蛋白，发现其通过激活p53依赖性通路诱导细胞周期停滞，抑制肿瘤细胞增殖。
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4. **文献名称**：*Crystal structure of BHLHB2/DEC1 reveals DNA-binding mechanism and dimerization interface*
**作者**：Nakashima, A., et al.
**摘要**：通过解析BHLHB2蛋白的晶体结构，阐明其结合靶基因DNA的分子机制及二聚化关键位点，为研究其转录调控功能提供结构基础。
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**注**：BHLHB2亦称DEC1、STRA13.属于碱性螺旋-环-螺旋(bHLH)转录因子家族，其重组蛋白常用于功能机制及疾病治疗相关研究。
Basic helix-loop-helix family member B2 (BHLHB2), also known as DEC1 or STRA13. is a transcription factor belonging to the class B basic helix-loop-helix (bHLH) superfamily. It plays multifaceted roles in cellular differentiation, circadian rhythm regulation, and tumorigenesis. Structurally, BHLHB2 contains a conserved bHLH domain essential for DNA binding and dimerization with other transcription regulators. Functionally, it modulates gene expression by either directly binding to E-box elements or interacting with transcriptional co-factors like HIF-1α and CLOCK/BMAL1 complexes.
In physiological contexts, BHLHB2 is involved in stress responses, energy metabolism, and neuronal differentiation. Its expression fluctuates under circadian control and is induced by hypoxia, DNA damage, and hormonal signals. Notably, BHLHB2 exhibits dual roles in cancer: acting as a tumor suppressor by promoting cell cycle arrest and apoptosis in certain contexts, while paradoxically driving cancer progression through epithelial-mesenchymal transition (EMT) and stemness enhancement in others. Dysregulation of BHLHB2 has been linked to multiple malignancies, including breast, lung, and colorectal cancers.
Recombinant BHLHB2 protein, typically produced in Escherichia coli or mammalian expression systems, enables mechanistic studies of its DNA-binding properties, post-translational modifications, and protein-protein interactions. Its applications span molecular pathway dissection, drug screening, and therapeutic targeting in circadian disorders and oncology research.
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