| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BENE |
| Uniprot No | Q13021 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MASPDPPATSYAPSDVPSGVALFLTIPFAFFLPELIFGFLVWTMVAATHIVYPLLQGWVMYVSLTSFLISLMFLLSYLFGFYKRFESWRVLDSLYHGTTGILYMSAAVLQVHATIVSEKLLDPRIYYINSAASFFAFIATLLYILHAFSIYYH |
| 分子量 | 42.57 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MAL样蛋白(MALL)相关的文献示例及摘要概括：
1. **文献名称**：*MALL, a membrane-associated protein required for cell polarization in epithelial cells*
**作者**：Martín-Belmonte F. et al.
**摘要**：研究揭示了MALL蛋白在上皮细胞极性形成中的作用，通过克隆人源重组MALL证明其通过调控脂筏结构域参与顶端膜运输。
2. **文献名称**：*The MAL proteolipid family in membrane microdomain organization*
**作者**：Segawa K. et al.
**摘要**：探讨了MALL蛋白作为MAL家族成员在细胞膜微区(如脂筏)中的分布特性，并通过重组蛋白实验验证其与膜运输相关蛋白的互作。
3. **文献名称**：*MALL gene expression and localization in human keratinocytes*
**作者**：Magal L.G. et al.
**摘要**：利用重组MALL转染实验，发现该蛋白在表皮细胞的分化过程中动态定位，并可能通过调控EGFR信号通路影响细胞增殖。
4. **文献名称**：*Tumor suppressor role of MALL in colorectal cancer*
**作者**：Gómez V. et al.
**摘要**：通过重组MALL过表达实验，证实其抑制结直肠癌细胞迁移和侵袭的机制，可能与抑制整合素信号通路相关。
(注：以上文献标题及作者为示例性虚构内容，具体研究需参考真实数据库如PubMed等检索结果。)
Re MAL-like protein (MALL), also known as MAL2. is a member of the MAL proteolipid family involved in membrane trafficking and cell polarization. It shares structural homology with MAL (T-cell differentiation protein), featuring four transmembrane domains (TMDs) and cytoplasmic N- and C-termini. MALL is ubiquitously expressed, prominently in epithelial cells, and localizes to cholesterol-rich lipid rafts, facilitating cargo sorting and vesicular transport between apical membranes and endosomes.
Functionally, MALL contributes to maintaining epithelial polarity by regulating apical protein delivery. Emerging studies link it to clathrin-independent endocytosis, particularly in hepatocyte bile transport. Dysregulation of MALL is implicated in tumorigenesis, showing altered expression in cancers like hepatocellular carcinoma and breast cancer. Overexpression may promote metastasis via membrane protein trafficking pathways, while downregulation disrupts cell-cell adhesion. Its role in cancer remains underexplored, but MALL is proposed as a potential biomarker or therapeutic target. Recent proteomic studies also suggest interactions with signaling proteins like EGFR, hinting at broader roles in cellular signaling. Despite progress, mechanistic details of MALL's trafficking regulation and disease associations require further validation, particularly in vivo models.
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