| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGO2 |
| Uniprot No | Q9UKV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 517-818aa |
| 氨基酸序列 | LVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLV |
| 预测分子量 | 36.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGO2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity*
**作者**：Elkayam, E., et al.
**摘要**：该研究解析了人源AGO2蛋白的晶体结构，揭示了其与miRNA结合的关键结构域及催化活性位点，为理解重组AGO2在RNA干扰中的分子机制提供了结构基础。
2. **文献名称**：*Human Argonaute2 Mediates RNA Cleavage Targeted by miRNAs and siRNAs*
**作者**：Meister, G., et al.
**摘要**：通过体外实验证明重组表达的人AGO2蛋白能直接介导siRNA或miRNA引导的靶RNA切割，证实其作为RISC复合体核心催化组分的功能活性。
3. **文献名称**：*Reconstitution of the MicroRNA Pathway with Recombinant Ago2 and GW182 Proteins*
**作者**：Höck, J., et al.
**摘要**：利用重组AGO2和GW182蛋白重建了哺乳动物miRNA通路，阐明AGO2通过招募GW182蛋白引发靶mRNA沉默的分子机制，验证了重组蛋白在功能研究中的应用价值。
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以上文献涵盖AGO2重组蛋白的结构解析、功能验证及通路重建研究，均为该领域的经典工作。
Argonaute-2 (AGO2) is a central component of the RNA-induced silencing complex (RISC), playing a critical role in post-transcriptional gene regulation through RNA interference (RNAi) and microRNA (miRNA) pathways. As a member of the Argonaute protein family, AGO2 binds small non-coding RNAs, such as small interfering RNAs (siRNAs) or miRNAs, to guide sequence-specific silencing of target mRNAs. Its catalytic activity, mediated by the conserved PIWI domain, enables endonucleolytic cleavage of complementary mRNA strands, a hallmark of RNAi.
Recombinant AGO2 protein is engineered to replicate this native functionality in vitro or ex vivo. Produced using heterologous expression systems (e.g., Escherichia coli, insect, or mammalian cells), recombinant AGO2 retains key structural features, including RNA-binding domains (PAZ and MID) and catalytic residues. However, post-translational modifications (e.g., phosphorylation) in mammalian-expressed versions often enhance its stability and activity.
Researchers employ recombinant AGO2 to dissect RISC assembly mechanisms, study miRNA-mediated gene regulation, and develop RNA-based therapeutics. Its applications extend to high-throughput screens for RNAi efficiency, CRISPR-Cas9-independent gene editing tools, and synthetic RISC platforms for targeted mRNA degradation. Challenges persist in maintaining its conformational integrity during purification and mimicking cellular conditions for functional studies.
Quality control metrics for recombinant AGO2 include RNA-binding capacity, cleavage activity assays, and absence of nucleases. As a versatile tool, it bridges basic research and translational innovation, particularly in oncology and antiviral therapies where precision gene silencing is paramount. Ongoing engineering aims to optimize its delivery, specificity, and compatibility with synthetic guide RNAs.
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