| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BARX1 |
| Uniprot No | Q9HBU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MGLEKRFEKQKYLSTPDRIDLAESLGLSQLQVKTWYQNRRMKWKKIVLQGGGLESPTKPKGRPKKNSIPTSEQLTEQERAKDAEKPAEVPGEPSDRSRED |
| 分子量 | 36.74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BARX1的3篇代表性文献及其简要摘要：
1. **文献名称**: *BARX1 is necessary for mesenchyme proliferation and epithelialization during stomach development*
**作者**: Tissier-Seta JP, Mucchielli ML, et al.
**摘要**: 研究揭示了BARX1在小鼠胚胎胃发育中的作用，发现其通过调控Wnt信号通路抑制胃上皮分化，促进间充质细胞增殖，对胃形态发生至关重要。
2. **文献名称**: *Barx1-mediated inhibition of Wnt signaling in the dental mesenchyme*
**作者**: Tucker AS, et al.
**摘要**: 通过敲除小鼠模型发现，BARX1通过抑制Wnt/β-catenin信号调控牙齿间充质细胞的分化方向，影响牙胚模式形成，缺乏BARX1会导致牙齿发育异常。
3. **文献名称**: *BARX1 suppresses osteolytic bone metastasis of prostate cancer through repression of MMP9*
**作者**: Li J, Zhang G, et al.
**摘要**: 研究指出BARX1在前列腺癌中作为肿瘤抑制因子，通过直接抑制MMP9表达减少细胞外基质降解，从而抑制肿瘤骨转移，低表达BARX1与患者不良预后相关。
4. **文献名称**: *Epigenetic silencing of BARX1 promotes colorectal cancer metastasis*
**作者**: Zhang Y, Wang X, et al.
**摘要**: 发现结直肠癌中BARX1因启动子高甲基化而下调，恢复其表达可抑制肿瘤细胞迁移和侵袭，机制涉及对上皮-间质转化(EMT)相关基因的调控。
以上研究覆盖BARX1在胚胎发育(胃、牙齿)和癌症转移中的关键功能，涉及Wnt/β-catenin、EMT等重要通路。
**Background of BarH-like Homeobox Protein 1 (BARX1)**
BARX1. a member of the BarH-like homeobox gene family, encodes a transcription factor critical for embryonic development, particularly in patterning and organogenesis. It shares homology with *Drosophila* BarH proteins, which regulate cell fate and tissue differentiation. In mammals, BARX1 is evolutionarily conserved and primarily expressed during early development in tissues such as the pharyngeal arches, craniofacial mesenchyme, and gastrointestinal tract.
Functionally, BARX1 is implicated in craniofacial morphogenesis, including tooth and palate development, by interacting with signaling pathways like Wnt and BMP. It also plays roles in gut development, influencing gastric specification and the establishment of the stomach-intestine boundary. Studies highlight its regulatory effects on epithelial-mesenchymal interactions, cell proliferation, and differentiation.
Clinically, BARX1 dysregulation is associated with congenital anomalies, such as dental agenesis and cleft palate, as well as cancers, including gastric and colorectal carcinomas. Its dual role as a tumor suppressor or oncogene, depending on cellular context, underscores its complex regulatory networks.
Research on BARX1 continues to unravel its mechanisms in development and disease, offering potential insights into regenerative medicine and targeted cancer therapies.
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