| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BARHL1 |
| Uniprot No | Q9BZE3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MEGSNGFGIDSILSHRAGSPALPKGDPLLGDCRSPLELSPRSESSSDCSSPASPGRDCLETGTPRPGGASGPGLDSHLQPGQLSAPAQSRTVTSSFLIRDILADCKPLAACAPYSSSGQPAAPEPGGRLAAKAAEDFRDKLDKSGSNASSDSEYKVKEEGDREISSSRDSPPVRLKKPRKARTAFTDHQLAQLERSFERQKYLSVQDRMELAASLNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYPQSLVSNLDPGAALYLYRGPSAPPPALQRPLVPRILIHGLQGASEPPPPLPPLAGVLPRAAQPR |
| 分子量 | 61.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BarH样1同源框蛋白(BARHL1)的参考文献及其摘要概括：
1. **文献名称**：*BARHL1 is required for maintenance of auditory neurons and maturation of the endocochlear potential*
**作者**：Duncan JS et al.
**摘要**：研究揭示了BARHL1在小鼠听觉系统发育中的作用，表明其缺失导致听觉神经元的退化和内耳电位异常，提示其对听觉功能维持至关重要。
2. **文献名称**：*BARHL1 regulates the differentiation of cerebellar granule cells through the Shh signaling pathway*
**作者**：Hosoya M et al.
**摘要**：发现BARHL1通过调控SHH(Sonic Hedgehog)信号通路参与小脑颗粒细胞的分化，其表达缺失会导致小鼠小脑发育异常。
3. **文献名称**：*Epigenetic silencing of BARHL1 promotes gastric cancer cell invasion and metastasis*
**作者**：Wang Y et al.
**摘要**：该研究表明，BARHL1在胃癌中因启动子高甲基化而被沉默，其下调激活Wnt/β-catenin通路，促进肿瘤侵袭和转移。
4. **文献名称**：*BARHL1 interacts with miRNA clusters to regulate retinal neuron differentiation*
**作者**：Bodea GO et al.
**摘要**：探讨BARHL1通过结合特定microRNA簇(如miR-9/124)调控视网膜神经元的分化，揭示其在神经特异性基因表达中的表观遗传调控作用。
以上文献涵盖BARHL1在神经发育、癌症及表观遗传调控中的关键功能，涉及听觉系统、小脑、胃癌及视网膜等研究模型。
**BarH-like homeobox protein 1 (BARHL1)** is a transcription factor belonging to the BarH-like family of homeodomain-containing proteins. It plays a critical role in developmental processes, particularly in the nervous system. Initially identified through homology with *Drosophila* BarH genes, BARHL1 is evolutionarily conserved and involved in cell fate determination, differentiation, and survival. It is prominently expressed during embryogenesis, notably in sensory organs (e.g., retina, inner ear) and regions of the brain, suggesting its importance in neural patterning and sensory organ development.
Structurally, BARHL1 contains a homeodomain responsible for DNA binding, enabling regulation of target genes. Studies link it to neurogenesis, where it influences progenitor cell differentiation and neuronal migration. Dysregulation of BARHL1 has been implicated in pathological conditions, including cancers such as breast cancer, glioblastoma, and neuroblastoma, where it may act as a tumor suppressor or context-dependent oncogene. Epigenetic silencing or mutations in BARHL1 correlate with poor prognosis, highlighting its potential as a biomarker.
Current research focuses on elucidating its precise molecular mechanisms, interactions with signaling pathways (e.g., Wnt, Notch), and therapeutic implications. While its role in neurodevelopmental disorders remains underexplored, BARHL1's conserved functions across species underscore its biological significance, offering avenues for understanding both normal development and disease.
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