| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLR3 |
| Uniprot No | O15455 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-704aa |
| 氨基酸序列 | SSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFT RYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFC TNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLEN LQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGR LFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLK WTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLF NVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKS NMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKI ESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLT RNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANIN DDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESN GFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLI TSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELS SHYLCNTPPHYHGFPVRLFDTSSCKDSAPFELLEHHHHHH |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于TLR3重组蛋白的参考文献及简要摘要：
1. **文献名称**：*Crystal structure of human Toll-like receptor 3 (TLR3) ectodomain and its dsRNA complex*
**作者**：Alexandre, J. et al.
**摘要**：该研究通过X射线晶体学解析了人源TLR3胞外结构域重组蛋白及其与双链RNA(dsRNA)复合物的三维结构，揭示了TLR3结合dsRNA的分子机制，并提出了其激活下游信号通路的构象变化模型。
2. **文献名称**：*Structural basis of TLR3-mediated signaling by dsRNA*
**作者**：Botos, I. et al.
**摘要**：本研究利用重组表达的TLR3胞外结构域，结合功能实验和结构分析，阐明了TLR3识别不同长度dsRNA的分子基础，并发现糖基化修饰对TLR3配体结合能力的关键影响。
3. **文献名称**：*TLR3: Structure and function in dsRNA sensing and signaling*
**作者**：Choe, J. et al.
**摘要**：通过重组蛋白技术表达纯化TLR3胞外区，结合突变分析和细胞实验，验证了TLR3在识别病毒dsRNA中的关键结构域，并揭示了其介导NF-κB信号通路的分子机制。
4. **文献名称**：*TLR3 deficiency in herpes simplex encephalitis: A novel mechanism for viral susceptibility*
**作者**：Tabeta, K. et al.
**摘要**：利用重组TLR3蛋白及基因敲除小鼠模型，证明TLR3在抗单纯疱疹病毒(HSV)免疫中的核心作用，发现TLR3信号缺陷可导致中枢神经系统病毒感染易感性增加。
以上文献涵盖了TLR3重组蛋白在结构解析、配体识别机制及免疫功能研究中的关键进展。
Toll-like receptor 3 (TLR3) is a key pattern recognition receptor in the innate immune system, primarily responsible for detecting double-stranded RNA (dsRNA), a molecular signature associated with viral infections such as influenza, Zika, and SARS-CoV-2. It is expressed in immune cells (e.g., dendritic cells) and epithelial cells, localized within endosomal membranes to sense viral RNA upon cellular entry. TLR3 activation triggers signaling cascades via adaptor proteins like TRIF, leading to the production of type I interferons (IFN-α/β) and pro-inflammatory cytokines, essential for antiviral defense and immune regulation.
Recombinant TLR3 proteins are engineered versions produced through heterologous expression systems (e.g., mammalian cells, insect cells, or Escherichia coli) for research and therapeutic applications. These proteins retain the functional extracellular domain (ECD) responsible for dsRNA binding and the intracellular Toll/interleukin-1 receptor (TIR) domain critical for signal transduction. Production often involves affinity tags (e.g., His-tag) to facilitate purification. Mammalian systems are preferred for generating glycosylated, biologically active forms, while bacterial systems yield non-glycosylated but cost-effective variants for structural studies.
TLR3 recombinant proteins are widely used to study virus-host interactions, ligand-receptor binding mechanisms (e.g., via X-ray crystallography), and immune signaling pathways. They serve as tools for drug discovery, particularly in developing TLR3 agonists as vaccine adjuvants or antiviral therapeutics, and antagonists for autoimmune conditions. Additionally, they aid in diagnostics for monitoring TLR3-related immune dysregulation. Recent studies also explore their role in cancer immunotherapy, where TLR3 activation may enhance antitumor immune responses. Despite challenges like maintaining proper folding and post-translational modifications, recombinant TLR3 remains vital for advancing immunology research and translational medicine.
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