关键信息
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基因名
RAB27B
- 应用
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别名
RAB27B;Ras-related Protein Rab-27B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00194
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表达区间
1-218aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMTDGDYDYLIKLLALGDSGVGKTTFLYRYT DNKFNPKFITTVGIDFREKRVVYNAQGPNGSSGKAFKVHLQLWDTAGQER FRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVL IGNKADLPDQREVNERQARELADKYGIPYFETSAATGQNVEKAVETLLDL IMKRMEQCVEKTQIPDTVNGGNSGNLDGEKPPEKKCIC
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分子量
27 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RAB27B is a member of the RAB family of small GTPases, which are pivotal in regulating intracellular transport processes, particularly in the exocytosis of secretory granules. This protein plays a crucial role in various cellular functions, including the secretion of hormones, neurotransmitters, and various biomolecules. Studies have shown that RAB27B is essential for the proper function of immune cells, such as mast cells and cytotoxic T lymphocytes, where it governs the release of inflammatory mediators and cytotoxic granules. Mutations or dysregulation of RAB27B have been linked to various diseases, including immunological disorders and cancer, highlighting its potential as a therapeutic target. The recombinant expression and functional characterization of RAB27B allow researchers to better understand its role in cellular mechanisms and its implications in disease pathology. The availability of RAB27B protein in a recombinant form facilitates structural studies and the identification of interacting partners, paving the way for the development of targeted therapies aimed at modulating its activity in diseases where its function is compromised. Overall, the exploration of RAB27B as a research subject holds promise for uncovering novel insights into intracellular trafficking and enhancing our understanding of related pathophysiological processes.
