| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CADM4 |
| Uniprot No | Q8NFZ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323aa |
| 氨基酸序列 | MGRARRFQWP LLLLWAAAAG PGAGQEVQTE NVTVAEGGVA EITCRLHQYD GSIVVIQNPA RQTLFFNGTR ALKDERFQLE EFSPRRVRIR LSDARLEDEG GYFCQLYTED THHQIATLTV LVAPENPVVE VREQAVEGGE VELSCLVPRS RPAATLRWYR DRKELKGVSS SQENGKVWSV ASTVRFRVDR KDDGGIIICE AQNQALPSGH SKQTQYVLDV QYSPTARIHA SQAVVREGDT LVLTCAVTGN PRPNQIRWNR GNESLPERAE AVGETLTLPG LVSADNGTYT CEASNKHGHA RALYVLVVYD PGAVVEAQTS VPY |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CADM4重组蛋白的3篇参考文献示例(注：内容为示例性虚构，仅供参考格式)：
1. **文献名称**：*Structural characterization of recombinant CADM4 and its role in neural cell adhesion*
**作者**：Zhang L, et al.
**摘要**：本研究通过大肠杆菌系统表达了重组CADM4蛋白，利用X射线晶体学解析其胞外段结构，揭示了其免疫球蛋白样结构域的关键结合位点，并证实其在神经元突触形成中的粘附功能。
2. **文献名称**：*CADM4 recombinant protein inhibits tumor metastasis via modulating EGFR signaling in non-small cell lung cancer*
**作者**：Tanaka K, et al.
**摘要**：通过哺乳动物细胞表达纯化的重组CADM4蛋白，研究发现其可竞争性抑制EGFR信号通路，降低肺癌细胞迁移能力，提示CADM4作为肿瘤抑制因子的潜在治疗价值。
3. **文献名称**：*Interaction between CADM4 and PDZ-domain proteins analyzed by recombinant protein pull-down assays*
**作者**：Wang Y, et al.
**摘要**：利用重组CADM4蛋白进行体外结合实验，发现其C末端与多种PDZ结构域蛋白(如DLG1)特异性结合，为研究CADM4在细胞极性建立中的分子机制提供了依据。
(注：实际研究中请通过学术数据库检索真实文献。)
**Background of CADM4 Recombinant Protein**
CADM4 (Cell Adhesion Molecule 4), also known as SynCAM4 or IGSF4C, is a member of the immunoglobulin (Ig) superfamily of cell adhesion molecules (CAMs). It plays a critical role in mediating cell-cell interactions through homophilic and heterophilic binding, contributing to tissue organization, synaptic connectivity, and signal transduction. Structurally, CADM4 contains extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail that interacts with intracellular scaffolding proteins, such as protein 4.1 family members, linking it to the cytoskeleton.
CADM4 is predominantly expressed in the nervous system, including neurons and glial cells, where it supports synaptic formation, maintenance, and plasticity. Beyond the brain, it is found in epithelial tissues, immune cells, and certain cancers, suggesting roles in cell polarity, immune regulation, and tumor suppression. Dysregulation of CADM4 has been implicated in neurological disorders, autoimmune diseases, and cancer progression, where its loss or altered expression may disrupt cell adhesion, promote metastasis, or impair neural communication.
Recombinant CADM4 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, functional protein for research and therapeutic applications. It retains the extracellular domain’s binding capacity, enabling studies on adhesion mechanisms, ligand-receptor interactions, and signaling pathways. Researchers use it to investigate CADM4’s role in neural development, tumor microenvironment modulation, and immune synapse formation. Additionally, it serves as a potential therapeutic agent or target for diseases linked to CADM4 dysfunction, such as neurodevelopmental disorders or cancers.
The production of recombinant CADM4 often involves tags (e.g., Fc, His) for purification and detection. Its applications span structural biology, drug screening, and functional assays, providing insights into cell adhesion dynamics and novel treatment strategies.
×
