| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPM1A |
| Uniprot No | P35813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382aa |
| 氨基酸序列 | GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW |
| 预测分子量 | 69.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPM1A重组蛋白的3条代表性文献示例(内容基于学术研究主题概括，非真实文献)：
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1. **文献名称**：*PPM1A dephosphorylates p38 MAPK to regulate stress response*
**作者**：Lin, J. et al.
**摘要**：研究通过表达重组人PPM1A蛋白，证实其在体外选择性去磷酸化激活的p38 MAPK，揭示了PPM1A通过负调控p38信号通路参与细胞应激反应的作用机制。
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2. **文献名称**：*Structural insights into the catalytic mechanism of recombinant PPM1A*
**作者**：Chen, X. & Wang, Y.
**摘要**：利用重组PPM1A蛋白进行晶体结构解析，揭示了其依赖镁离子的磷酸酶活性中心构象，为设计靶向PPM1A的小分子抑制剂提供了结构基础。
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3. **文献名称**：*PPM1A inhibits AMPK signaling via direct dephosphorylation of T172*
**作者**：Smith, A. et al.
**摘要**：通过体外重组蛋白实验证明，PPM1A可直接作用于AMPKα亚基，去磷酸化其Thr172位点，从而负调控AMPK活性，影响细胞能量代谢平衡。
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(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索。)
PPM1A (Protein Phosphatase Mg²⁺/Mn²⁺ Dependent 1A), also known as PP2Cα, is a member of the metal-dependent protein phosphatase PPM family. It plays a critical role in regulating cellular signaling pathways by dephosphorylating serine/threonine residues on target proteins, counteracting kinase-mediated phosphorylation. PPM1A is involved in diverse biological processes, including stress response, cell cycle progression, apoptosis, and metabolic regulation. Key substrates include components of the TGF-β, AMPK, and p38 MAPK pathways, linking it to cancer, diabetes, and inflammatory diseases. Dysregulation of PPM1A has been implicated in tumor suppression or promotion, depending on context, highlighting its dual role in cellular homeostasis.
Recombinant PPM1A protein is engineered for in vitro studies to investigate its enzymatic activity, substrate specificity, and interaction networks. It is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) using genetic engineering techniques, ensuring high purity and biological activity through affinity chromatography. Researchers utilize this tool to explore PPM1A's regulatory mechanisms in disease models, screen for modulators (inhibitors/activators), and validate its therapeutic potential. Its recombinant form enables precise control in biochemical assays, structural studies (e.g., crystallography), and functional characterization, advancing our understanding of phosphatase biology and drug discovery efforts targeting PPM1A-related pathologies.
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