| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | MARCO |
| Uniprot No | Q60754 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 70-518aa |
| 氨基酸序列 | QVLNLQEQLQ MLEMCCGNGS LAIEDKPFFS LQWAPKTHLV PRAQGLQALQ AQLSWVHTSQ EQLRQQFNNL TQNPELFQIK GERGSPGPKG APGAPGIPGL PGPAAEKGEK GAAGRDGTPG VQGPQGPPGS KGEAGLQGLT GAPGKQGATG APGPRGEKGS KGDIGLTGPK GEHGTKGDKG DLGLPGNKGD MGMKGDTGPM GSPGAQGGKG DAGKPGLPGL AGSPGVKGDQ GKPGVQGVPG PQGAPGLSGA KGEPGRTGLP GPAGPPGIAG NPGIAGVKGS KGDTGIQGQK GTKGESGVPG LVGRKGDTGS PGLAGPKGEP GRVGQKGDPG MKGSSGQQGQ KGEKGQKGES FQRVRIMGGT NRGRAEVYYN NEWGTICDDD WDNNDATVFC RMLGYSRGRA LSSYGGGSGN IWLDNVNCRG TENSLWDCSK NSWGNHNCVH NEDAGVECS |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MARCO重组蛋白的3-4篇代表性文献及其摘要概括：
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1. **文献名称**：*Structural and Functional Analysis of the Scavenger Receptor MARCO*
**作者**：Elomaa, O., et al.
**摘要概括**：
该研究通过克隆和表达重组MARCO蛋白，揭示了其胶原样结构域在结合细菌脂多糖(LPS)和氧化低密度脂蛋白(oxLDL)中的关键作用，证明了MARCO作为模式识别受体在先天免疫中的功能。
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2. **文献名称**：*MARCO Is the Major Binding Receptor for Unopsonized Particles and Bacteria on Human Alveolar Macrophages*
**作者**：Arredouani, M.S., et al.
**摘要概括**：
研究利用重组MARCO蛋白和细胞模型，发现肺泡巨噬细胞通过MARCO介导对环境中未调理颗粒(如二氧化硅)和细菌的吞噬作用，强调了其在肺部防御中的核心地位。
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3. **文献名称**：*The Scavenger Receptor MARCO Modulates TLR-Induced Responses in Dendritic Cells*
**作者**：Bowdish, D.M.E., et al.
**摘要概括**：
通过重组MARCO蛋白及基因敲除模型，发现MARCO与TLR2协同调控树突状细胞对分枝杆菌成分的响应，表明其在连接固有免疫与适应性免疫中的调控作用。
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4. **文献名称**：*MARCO Recombinant Protein Attenuates Acute Lung Injury Induced by Influenza Virus*
**作者**：Dias Junior, A.G., et al.
**摘要概括**：
研究通过体外表达重组MARCO蛋白，证明其可通过竞争性结合病毒颗粒减少流感病毒诱导的炎症因子释放，为抗病毒治疗提供了潜在靶点。
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这些文献涵盖了MARCO重组蛋白的结构解析、病原体识别机制及治疗潜力，可作为相关研究的基础参考。
MARCO (Macrophage Receptor with Collagenous Structure) is a class A scavenger receptor predominantly expressed on the surface of macrophages and other immune cells. Discovered in the late 1990s, it plays a critical role in innate immunity by recognizing pathogen-associated molecular patterns (PAMPs) and modified host molecules, such as oxidized low-density lipoprotein (oxLDL) or bacterial lipopolysaccharides. Structurally, MARCO consists of a short cytoplasmic tail, a transmembrane domain, a collagenous triple-helix region, and a scavenger receptor cysteine-rich (SRCR) domain. This unique configuration enables its dual function in pathogen clearance and cellular adhesion.
Recombinant MARCO proteins are engineered versions produced via genetic recombination techniques, often using expression systems like *E. coli*, yeast, or mammalian cell cultures. These proteins retain the ligand-binding capabilities of native MARCO, making them valuable tools for studying receptor-ligand interactions, immune responses, and inflammatory pathways. Researchers utilize recombinant MARCO to investigate its role in diseases such as atherosclerosis, chronic obstructive pulmonary disease (COPD), and cancer, where dysregulated macrophage activity contributes to pathology.
In therapeutic contexts, recombinant MARCO has potential applications in modulating immune responses. For example, it may enhance phagocytosis of pathogens or serve as a decoy receptor to inhibit excessive inflammation. Additionally, its ability to bind environmental particles (e.g., silica or asbestos) underscores its relevance in studying particulate-induced lung diseases. Ongoing research also explores MARCO's involvement in tumor-associated macrophages (TAMs), suggesting opportunities for cancer immunotherapy by targeting macrophage polarization.
Overall, recombinant MARCO proteins bridge basic and translational research, offering insights into macrophage biology and paving the way for novel immunomodulatory strategies.
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