| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASGPR1 |
| Uniprot No | P07306 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 62-291aa |
| 氨基酸序列 | QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEK QQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEH ERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTW MGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDD GRWNDDVCQRPYRWVCETELDKASQEPPLLVDHHHHHH |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASGPR1重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**：*Expression and functional characterization of recombinant human asialoglycoprotein receptor 1 (ASGPR1)*
**作者**：Smith A, et al.
**摘要**：研究报道了人源ASGPR1重组蛋白在哺乳动物细胞中的高效表达与纯化，验证其与半乳糖化配体的特异性结合能力，并探讨其在肝靶向药物递送中的应用潜力。
2. **文献名称**：*Structural insights into ligand recognition by the ASGPR1 receptor*
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析了重组ASGPR1的胞外结构域三维结构，揭示了其与半乳糖配体结合的关键氨基酸残基，为靶向药物设计提供结构基础。
3. **文献名称**：*Recombinant ASGPR1-mediated delivery of siRNA for liver-specific gene silencing*
**作者**：Wang Y, et al.
**摘要**：利用重组ASGPR1蛋白修饰脂质纳米颗粒，实现siRNA的肝细胞特异性靶向递送，在动物模型中显著降低目标基因表达，展示其在基因治疗中的应用价值。
(注：上述文献为示例性内容，实际引用需根据具体研究补充真实文献信息。)
**Background of ASGPR1 Recombinant Protein**
The asialoglycoprotein receptor 1 (ASGPR1), also known as the hepatic lectin, is a C-type lectin receptor predominantly expressed on the sinusoidal surface of hepatocytes. It plays a critical role in the clearance of desialylated glycoproteins from circulation by binding to terminal galactose or N-acetylgalactosamine residues. Structurally, ASGPR1 is a hetero-oligomeric complex composed of two homologous subunits, H1 and H2. encoded by distinct genes (ASGR1 and ASGR2). These subunits form a functional receptor through disulfide linkages, enabling high-affinity ligand recognition and endocytosis.
Recombinant ASGPR1 protein is engineered in vitro using expression systems (e.g., mammalian or insect cells) to mimic the native receptor's ligand-binding properties. It retains the extracellular carbohydrate-recognition domain (CRD), essential for glycan interaction, and is often purified for functional studies. This recombinant protein serves as a vital tool for investigating ASGPR1-mediated endocytosis, glycoprotein metabolism, and liver-targeted drug delivery mechanisms.
ASGPR1 has garnered significant interest in therapeutic applications due to its liver-specific expression. It is exploited for hepatocyte-directed delivery of drugs, nucleic acids (e.g., siRNA), or gene therapies, enhancing tissue specificity while minimizing off-target effects. Additionally, recombinant ASGPR1 aids in studying liver diseases, such as viral hepatitis and hepatocellular carcinoma, where receptor dysfunction or altered expression may contribute to pathogenesis. Its role in immune modulation and antigen presentation is also under exploration.
Overall, ASGPR1 recombinant protein bridges fundamental research and translational medicine, offering insights into liver biology and enabling innovative therapeutic strategies.
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