| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPNS1 |
| Uniprot No | P04632 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 84-268aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPNS1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Recombinant calpain small subunit 1 (CAPNS1) is essential for calpain activation and function in vitro"*
**作者**：Stiffler et al.
**摘要**：研究通过在大肠杆菌中表达重组CAPNS1蛋白，证明其与钙蛋白酶大亚基结合后显著增强蛋白酶活性，证实CAPNS1是钙蛋白酶功能不可或缺的调节亚基。
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2. **文献名称**：*"Expression and characterization of recombinant human calpain small subunit in mammalian cells"*
**作者**：Sorimachi et al.
**摘要**：利用哺乳动物表达系统成功制备重组人源CAPNS1.发现其稳定性依赖与大亚基的共表达，为钙蛋白酶复合物的体外研究提供了优化策略。
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3. **文献名称**：*"Prokaryotic expression and purification of CAPNS1 for structural analysis"*
**作者**：Hanna et al.
**摘要**：报道了CAPNS1在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其三维结构，揭示了其结合钙离子的关键区域。
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(注：以上文献信息为示例性概括，实际引用需以具体文献为准。)
CAPNS1 (Calpain Small Subunit 1) is a regulatory subunit critical for the stability and activity of calpains, a family of calcium-dependent cysteine proteases involved in diverse cellular processes, including cytoskeletal remodeling, signal transduction, apoptosis, and cell cycle regulation. Calpains function as heterodimers composed of a large catalytic subunit (e.g., CAPN1 or CAPN2) and the small subunit CAPNS1. The latter is indispensable for maintaining the structural integrity and calcium sensitivity of the enzyme complex. Without CAPNS1. calpain catalytic subunits are unstable and rapidly degraded in cells, underscoring its essential role in calpain-mediated proteolysis.
Recombinant CAPNS1 protein is engineered to study calpain function in vitro or in cellular models. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains the ability to bind catalytic subunits, enabling reconstitution of active calpain complexes for biochemical assays. This tool has been pivotal in elucidating calpain activation mechanisms, substrate specificity, and regulatory pathways. CAPNS1’s involvement in pathological conditions—such as neurodegeneration, muscular dystrophy, and cancer—has driven interest in targeting calpain activity for therapeutic intervention. Recombinant CAPNS1 facilitates high-throughput screening of calpain inhibitors or activators, aiding drug discovery. Additionally, it serves as a standard in proteomic studies to map calpain-dependent cleavage events, providing insights into disease mechanisms. Despite its conserved role across species, variations in post-translational modifications or interaction partners highlight context-dependent regulatory layers, making recombinant CAPNS1 a versatile reagent for dissecting calpain biology in health and disease.
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