| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGFBR3 |
| Uniprot No | Q03167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-851aa |
| 氨基酸序列 | MTSHYVIAIFALMSSCLATAGPEPGALCELSPVSASHPVQALMESFTVLSGCASRGTTGLPQEVHVLNLRTAGQGPGQLQREVTLHLNPISSVHIHHKSVVFLLNSPHPLVWHLKTERLATGVSRLFLVSEGSVVQFSSANFSLTAETEERNFPHGNEHLLNWARKEYGAVTSFTELKIARNIYIKVGEDQVFPPKCNIGKNFLSLNYLAEYLQPKAAEGCVMSSQPQNEEVHIIELITPNSNPYSAFQVDITIDIRPSQEDLEVVKNLILILKCKKSVNWVIKSFDVKGSLKIIAPNSIGFGKESERSMTMTKSIRDDIPSTQGNLVKWALDNGYSPITSYTMAPVANRFHLRLENNAEEMGDEEVHTIPPELRILLDPGALPALQNPPIRGGEGQNGGLPFPFPDISRRVWNEEGEDGLPRPKDPVIPSIQLFPGLREPEEVQGSVDIALSVKCDNEKMIVAVEKDSFQASGYSGMDVTLLDPTCKAKMNGTHFVLESPLNGCGTRPRWSALDGVVYYNSIVIQVPALGDSSGWPDGYEDLESGDNGFPGDMDEGDASLFTRPEIVVFNCSLQQVRNPSSFQEQPHGNITFNMELYNTDLFLVPSQGVFSVPENGHVYVEVSVTKAEQELGFAIQTCFISPYSNPDRMSHYTIIENICPKDESVKFYSPKRVHFPIPQADMDKKRFSFVFKPVFNTSLLFLQCELTLCTKMEKHPQKLPKCVPPDEACTSLDASIIWAMMQNKKTFTKPLAVIHHEAESKEKGPSMKEPNPISPPIFHGLDTLTVMGIAFAAFVIGALLTGALWYIYSHTGETAGRQQVPTSPPASENSSAAHSIGSTQSTPCSSSSTA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"TGFBR3 (betaglycan) as a potent regulator of TGF-β signaling in cancer progression"**
- 作者：E. Derynck 等
- 摘要：探讨TGFBR3重组蛋白作为TGF-β共受体在肿瘤微环境中的作用，揭示其通过调控TGF-β1和TGF-β2的活性抑制肿瘤转移的机制。
2. **"Soluble TGFBR3-Fc fusion protein suppresses angiogenesis and tumor growth"**
- 作者：J. Lee 等
- 摘要：研究可溶性TGFBR3重组蛋白(融合Fc段)在体内模型中阻断TGF-β信号通路，抑制血管生成和乳腺癌生长的治疗效果。
3. **"Recombinant TGFBR3 extracellular domain attenuates pulmonary fibrosis via modulating Smad2/3 phosphorylation"**
- 作者：M. Zhang 等
- 摘要：证明TGFBR3重组胞外结构域通过竞争性结合TGF-β配体，减轻小鼠肺纤维化模型中Smad通路过度激活，提出其作为抗纤维化治疗策略的潜力。
4. **"Betaglycan (TGFBR3) enhances inhibin signaling by presenting ligands to ACVR2A in ovarian granulosa cells"**
- 作者：K. Wiater 等
- 摘要：揭示TGFBR3重组蛋白通过介导抑制素(inhibin)与ACVR2A受体的结合，调控卵巢颗粒细胞分化及生殖激素分泌的分子机制。
(注：以上文献为示例，实际引用需根据具体研究补充完整信息。)
**Background of TGFBR3 Recombinant Protein**
TGFBR3 (transforming growth factor-beta receptor type 3), also known as betaglycan, is a transmembrane proteoglycan that functions as a co-receptor in the TGF-β signaling pathway. This receptor binds TGF-β ligands, including TGF-β1. TGF-β2. and inhibins, facilitating their interactions with signaling receptors (TGFBR1 and TGFBR2) or modulating ligand availability. Structurally, TGFBR3 contains a large extracellular domain with heparan sulfate and chondroitin sulfate glycosaminoglycan chains, which enhance ligand binding and regulate cellular responses.
Unlike TGFBR1 and TGFBR2. TGFBR3 lacks intrinsic kinase activity but plays a critical role in fine-tuning TGF-β signaling. It can either promote or inhibit downstream pathways (e.g., SMAD or non-SMAD signaling), depending on cellular context. In some tissues, TGFBR3 acts as a "sink" by sequestering ligands, thereby attenuating signaling. Its soluble form (sTGFBR3), generated via ectodomain shedding, exhibits similar ligand-trapping functions and is implicated in pathological conditions like cancer and fibrosis.
Recombinant TGFBR3 proteins are engineered to mimic the extracellular domain, enabling studies on TGF-β pathway regulation. Produced in mammalian or insect expression systems, these proteins retain post-translational modifications essential for ligand binding. They are widely used in research to neutralize TGF-β activity, investigate receptor-ligand dynamics, or explore therapeutic strategies targeting TGF-β-driven diseases. Dysregulation of TGFBR3 expression is linked to tumor progression, cardiovascular disorders, and tissue fibrosis, underscoring its therapeutic potential. Recombinant TGFBR3 thus serves as a valuable tool for dissecting TGF-β biology and developing targeted interventions.
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