| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CADM3 |
| Uniprot No | Q8N126 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-330aa |
| 氨基酸序列 | NLSQDDSQPWTSDETVVAGGTVVLKCQVKDHEDSSLQWSNPAQQTLYFGE KRALRDNRIQLVTSTPHELSISISNVALADEGEYTCSIFTMPVRTAKSLV TVLGIPQKPIITGYKSSLREKDTATLNCQSSGSKPAARLTWRKGDQELHG EPTRIQEDPNGKTFTVSSSVTFQVTREDDGASIVCSVNHESLKGADRSTS QRIEVLYTPTAMIRPDPPHPREGQKLLLHCEGRGNPVPQQYLWEKEGSVP PLKMTQESALIFPFLNKSDSGTYGCTATSNMGSYKAYYTLNVNDPSPVPS SSSTYHVDHHHHHH |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CADM3重组蛋白的3篇参考文献示例(注：以下文献为假设性示例，实际引用需核实真实来源)：
1. **《Cloning and Functional Characterization of Recombinant CADM3 in Synaptic Adhesion》**
- **作者**: Yamamoto, K. et al.
- **摘要**: 本研究成功克隆并表达了人源CADM3重组蛋白，证实其在体外促进神经元突触前分化的功能，揭示了CADM3通过同源相互作用介导细胞间粘附的分子机制。
2. **《Structural Analysis of CADM3 Ig Domains and Implications for Axon Guidance》**
- **作者**: Chen, L. & Park, J.
- **摘要**: 通过X射线晶体学解析了CADM3重组蛋白的免疫球蛋白样结构域，发现其与神经轴突导向蛋白Netrin-1的相互作用，为理解神经系统发育提供了结构基础。
3. **《CADM3 Recombinant Protein Suppresses Tumor Metastasis via Cell-Cell Contact Inhibition》**
- **作者**: Gupta, R. et al.
- **摘要**: 研究表明，外源性添加CADM3重组蛋白可抑制肺癌细胞迁移和侵袭，机制涉及增强细胞间连接并下调EMT(上皮-间质转化)相关信号通路。
如需真实文献，建议在PubMed或Web of Science以“CADM3 recombinant protein”为关键词检索，并筛选近年研究。
CADM3 (Cell Adhesion Molecule 3), also known as Necl-3 or SynCAM3. is a transmembrane protein belonging to the immunoglobulin superfamily. It plays critical roles in cell-cell adhesion, synaptic organization, and intracellular signaling. Structurally, CADM3 contains three extracellular immunoglobulin-like domains, a single transmembrane region, and a cytoplasmic tail that interacts with scaffolding proteins like CASK. It is predominantly expressed in the nervous system, where it facilitates synapse formation, axonal guidance, and myelination by mediating homophilic or heterophilic interactions with other adhesion molecules like CADM1 or CADM2.
Recombinant CADM3 protein is engineered using expression systems (e.g., HEK293 cells, E. coli) to produce soluble forms of its extracellular domain for functional studies. This recombinant protein retains binding capacity, enabling researchers to investigate its role in neural development, tumor suppression, and immune modulation. Notably, CADM3 has been implicated in cancer biology, as its downregulation is associated with metastasis in certain malignancies. In neurodegenerative contexts, altered CADM3 expression correlates with synaptic dysfunction. Researchers utilize recombinant CADM3 to explore therapeutic targets, develop diagnostic tools, or model protein-protein interaction networks in vitro. Its applications span neurobiology, oncology, and regenerative medicine, offering insights into disease mechanisms and potential interventions.
×
