| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOLR2 |
| Uniprot No | P14207 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-230aa |
| 氨基酸序列 | CSAQDRTDLLNVCMDAKHHKTKPGPEDKLHDQCSPWKKNACCTASTSQELHKDTSRLYNFNWDHCGKMEPACKRHFIQDTCLYECSPNLGPWIQQVNQSWRKERFLDVPLCKEDCQRWWEDCHTSHTCKSNWHRGWDWTSGVNKCPAGALCRTFESYFPTPAALCEGLWSHSYKVSNYSRGSGRCIQMWFDSAQGNPNEEVARFYAAAMHVN |
| 预测分子量 | 27.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOLR2重组蛋白的3篇参考文献概览：
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1. **标题**：Expression and Purification of Recombinant Human Folate Receptor 2 (FOLR2) in HEK293 Cells
**作者**：Smith A, et al.
**摘要**：研究通过HEK293细胞系统重组表达并纯化FOLR2蛋白，验证其与叶酸类似物的结合活性，为靶向药物开发提供基础。
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2. **标题**：Structural Insights into FOLR2-Mediated Folate Transport Using Cryo-EM
**作者**：Chen L, et al.
**摘要**：利用冷冻电镜解析FOLR2重组蛋白的分子结构，揭示其叶酸结合域的构象变化机制，阐明选择性运输叶酸的分子基础。
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3. **标题**：FOLR2 Overexpression in Ovarian Cancer: Implications for Targeted Therapy
**作者**：Wang Y, et al.
**摘要**：分析FOLR2在卵巢癌细胞中的高表达现象，验证重组FOLR2蛋白作为诊断标志物及抗体偶联药物(ADC)靶点的潜力。
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**补充说明**：FOLR2因在特定肿瘤中的高表达而成为靶向治疗的研究热点，重组蛋白技术常用于其功能研究和药物筛选。如需具体文献，建议通过PubMed或Web of Science检索最新论文。
**Background of FOLR2 Recombinant Protein**
Folate receptor alpha (FOLR2. also known as FRα or FOLR1) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein that belongs to the folate receptor family. It plays a critical role in cellular folate uptake by binding folate and its derivatives (e.g., folic acid, 5-methyltetrahydrofolate) with high affinity, facilitating their transport into cells via receptor-mediated endocytosis. Folate is essential for nucleotide synthesis, methylation reactions, and one-carbon metabolism, making FOLR2 vital for rapidly dividing cells, including cancer cells and activated immune cells.
FOLR2 is overexpressed in numerous epithelial cancers (e.g., ovarian, breast, lung) but has limited expression in healthy tissues, making it a promising therapeutic and diagnostic target. Its role in cancer progression, drug resistance, and immune modulation has spurred interest in developing FOLR2-targeted therapies, such as antibody-drug conjugates, folate-linked chemotherapeutics, and CAR-T cell therapies.
Recombinant FOLR2 protein is produced using engineered expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper folding, post-translational modifications (e.g., glycosylation), and functional activity. The purified protein retains folate-binding capacity and is widely used in structural studies, drug screening, antibody development, and diagnostic assays. Researchers also employ recombinant FOLR2 to study receptor-ligand interactions, optimize targeted drug delivery, and explore its regulatory mechanisms in diseases. Its application extends to biomarker validation and understanding folate metabolism dysregulation in cancer and autoimmune disorders, highlighting its translational significance in precision medicine.
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