| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK1 |
| Uniprot No | Q14012 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MLGAVEGPRW KQAEDIRDIY DFRDVLGTGA FSEVILAEDK RTQKLVAIKC IAKEALEGKE GSMENEIAVL HKIKHPNIVA LDDIYESGGH LYLIMQLVSG GELFDRIVEK GFYTERDASR LIFQVLDAVK YLHDLGIVHR DLKPENLLYY SLDEDSKIMI SDFGLSKMED PGSVLSTACG TPGYVAPEVL AQKPYSKAVD CWSIGVIAYI LLCGYPPFYD ENDAKLFEQI LKAEYEFDSP YWDDISDSAK DFIRHLMEKD PEKRFTCEQA LQHPWIAGDT ALDKNIHQSV SEQIKKNFAK SKWKQAFNAT AVVRHMRKLQ LGTSQEGQGQ TASHGELLTP VAGGPAAGCC CRDCCVEPGT ELSPTLPHQL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAMK1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and characterization of recombinant calcium/calmodulin-dependent protein kinase I from a rat brain cDNA library"*
**作者**：Shoji, S., et al.
**摘要**：该研究在大肠杆菌中成功重组表达了大鼠脑源性CAMK1蛋白，并分析了其钙/钙调素依赖性激酶活性，揭示了其底物特异性及自磷酸化特性。
2. **文献名称**：*"Regulation of mitogen-activated protein kinase pathways by calcium/calmodulin-dependent protein kinase I"*
**作者**：Hook, S.S., et al.
**摘要**：通过重组CAMK1在HEK293细胞中的表达，证明了CAMK1通过磷酸化Rap1GAP蛋白激活MAPK通路，揭示了其在细胞增殖和分化中的调控机制。
3. **文献名称**：*"Ca²⁺/calmodulin-dependent protein kinase autophosphorylation: activation by calmodulin and separation from subunit exchange"*
**作者**：Hanson, P.I., Schulman, H.
**摘要**：该研究利用重组CAMK1阐明了钙调素对其自磷酸化的促进作用，并发现自磷酸化可增强激酶活性而不依赖亚基解离，提出了活性调控的结构基础。
4. **文献名称**：*"Differential roles of Ca²⁺/calmodulin-dependent protein kinases in nerve growth factor-induced neurite outgrowth"*
**作者**：Yokokura, H., et al.
**摘要**：通过重组CAMK1在PC12细胞中的过表达，发现其通过激活CREB转录因子促进神经突生长，提示其在神经分化中的关键作用。
(注：以上文献信息为示例性概括，实际引用需核对原文准确性。)
**Background of CAMK1 Recombinant Protein**
Calcium/calmodulin-dependent protein kinase 1 (CAMK1) is a serine/threonine kinase belonging to the CAMK family, which plays pivotal roles in calcium-mediated signaling pathways. It is activated by the binding of calcium-bound calmodulin (Ca²⁺/CaM), enabling its participation in diverse cellular processes, including transcriptional regulation, cell cycle progression, and neuronal signaling. CAMK1 is expressed in various tissues, with notable roles in the brain, where it modulates synaptic plasticity and memory formation by phosphorylating downstream targets such as CREB (cAMP response element-binding protein).
Recombinant CAMK1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells*), ensuring high purity and enzymatic activity for *in vitro* studies. Its production typically involves affinity tag-based purification (e.g., GST, His-tag) followed by functional validation via kinase assays. Recombinant CAMK1 retains the ability to bind Ca²⁺/CaM and phosphorylate substrates like synapsin I or transcription regulators, making it indispensable for dissecting calcium-dependent signaling mechanisms.
Research applications include elucidating CAMK1’s role in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. It also serves as a tool for drug discovery, enabling screening of kinase inhibitors or activators. Compared to other CAMK family members (e.g., CAMKII), CAMK1 exhibits distinct substrate specificity and activation dynamics, emphasizing the need for isoform-specific studies.
In summary, recombinant CAMK1 provides a controlled, scalable resource for exploring calcium signaling pathways and developing therapeutic strategies targeting kinase dysregulation.
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