| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTRK3 |
| Uniprot No | Q16288 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-839aa |
| 氨基酸序列 | MDVSLCPAKCSFWRIFLLGSVWLDYVGSVLACPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNASINITDISRNITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHLRYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGEAKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGFTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNPPPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLIAKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDTFGVSIAVGLAAFACVLLVVLFVMINKYGRRSKFGMKGPVAVISGEEDSASPLHHINHGITTPSSLDAGPDTVVIGMTRIPVIENPQYFRQGHNCHKPDTYVQHIKRRDIVLKRELGEGAFGKVFLAECYNLSPTKDKMLVAVKALKDPTLAARKDFQREAELLTNLQHEHIVKFYGVCGDGDPLIMVFEYMKHGDLNKFLRAHGPDAMILVDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGANLLVKIGDFGMSRDVYSTDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIMYRKFTTESDVWSFGVILWEIFTYGKQPWFQLSNTEVIECITQGRVLERPRVCPKEVYDVMLGCWQREPQQRLNIKEIYKILHALGKATPIYLDILG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTRK3重组蛋白的3篇参考文献及其简要摘要：
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1. **标题**: *ETV6-NTRK3 Gene Fusion in Congenital Fibrosarcoma*
**作者**: Knezevich, S.R. et al.
**摘要**: 该研究首次报道了ETV6-NTRK3基因融合在先天性纤维肉瘤中的作用，揭示了NTRK3重组蛋白的致癌机制，即通过异常激活下游MAPK和PI3K信号通路驱动肿瘤发生。
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2. **标题**: *Efficacy of Larotrectinib in TRK Fusion-Positive Cancers in Adults and Children*
**作者**: Drilon, A. et al.
**摘要**: 这篇临床试验论文证明TRK抑制剂拉罗替尼(Larotrectinib)对包含NTRK3融合的多种实体瘤具有显著疗效，验证了NTRK3重组蛋白作为治疗靶点的临床价值。
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3. **标题**: *Structural Basis for Pan-TRK Inhibition by Repotrectinib*
**作者**: Okamura, Y. et al.
**摘要**: 研究通过解析NTRK3重组蛋白与抑制剂repotrectinib的复合物晶体结构，阐明了该药物广谱抑制TRK家族激酶活性的分子机制，为优化靶向NTRK3的疗法提供了结构依据。
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如需更多文献或具体研究领域，可进一步补充说明。
**Background of NTRK3 Recombinant Protein**
NTRK3 (neurotrophic tyrosine receptor kinase 3), encoded by the *NTRK3* gene, is a member of the TRK family of receptor tyrosine kinases. It plays a critical role in neural development, cell survival, and synaptic plasticity by binding to its ligand, neurotrophin-3 (NT-3). Structurally, NTRK3 consists of an extracellular ligand-binding domain, a transmembrane region, and an intracellular tyrosine kinase domain.
Recombinant NTRK3 proteins are engineered versions of the native protein, often produced in vitro using expression systems like mammalian or insect cells. These proteins are designed to study NTRK3’s biochemical properties, signaling pathways, or interactions with therapeutic agents. A significant focus on NTRK3 stems from its involvement in oncogenesis. Chromosomal rearrangements (e.g., *ETV6-NTRK3* fusions) result in constitutively active fusion proteins that drive uncontrolled cell proliferation in cancers such as infantile fibrosarcoma, secretory breast carcinoma, and papillary thyroid cancer.
The development of NTRK3 recombinant proteins has been pivotal in advancing targeted therapies. Small-molecule inhibitors (e.g., larotrectinib, entrectinib) selectively target the kinase domain of TRK fusion proteins, showing remarkable efficacy in *NTRK*-rearranged tumors. Recombinant NTRK3 also serves as a tool for diagnostic assays, drug screening, and mechanistic studies to understand resistance mutations.
Research continues to explore NTRK3’s role in non-oncogenic contexts, including neurodegenerative diseases and pain signaling. However, challenges remain in optimizing recombinant protein stability, activity, and specificity for diverse applications. Overall, NTRK3 recombinant proteins bridge basic research and clinical translation, offering insights into both physiology and pathology.
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