| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFASC |
| Uniprot No | O94856 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1239-1347aa |
| 氨基酸序列 | KRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA |
| 预测分子量 | 15.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFASC重组蛋白的3篇代表性文献参考(注：内容基于领域知识模拟，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Neurofascin regulates axon initial segment assembly and function in vivo*
**作者**：Zonta, B., et al.
**摘要**：该研究利用重组NFASC蛋白体外验证了其在神经元轴突起始段(AIS)组装中的关键作用，揭示其通过与细胞骨架蛋白Ankyrin G的相互作用维持AIS结构和电信号传导功能。
2. **文献名称**：*Autoantibodies to nodal/paranodal proteins in neuropathic disorders*
**作者**：Devaux, J.J., et al.
**摘要**：研究利用重组NFASC蛋白作为抗原，检测多发性硬化症等患者血清中的自身抗体，证实抗NFASC抗体会破坏神经节点结构，导致传导阻滞和运动障碍。
3. **文献名称**：*Structural basis of neurofascin binding to gliomedin in the PNS nodal complex*
**作者**：Eshed-Eisenbach, Y., et al.
**摘要**：通过X射线晶体学解析重组NFASC胞外域与胶质细胞蛋白Gliomedin的复合物结构，阐明其在周围神经系统髓鞘形成中的分子互作机制。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词“NFASC recombinant protein”或“Neurofascin expression”获取最新研究。
Neurofascin (NFASC) is a member of the L1 immunoglobulin superfamily of cell adhesion molecules, primarily expressed in the nervous system. It plays critical roles in axonal guidance, neuronal-glial interactions, and the organization of specialized membrane domains, such as nodes of Ranvier and synaptic junctions. The NFASC gene encodes multiple splice variants, including NF186 (neuronal isoform) and NF155 (glial isoform), which differ in their extracellular domains and cellular localization. NF186 anchors voltage-gated sodium channels at nodes of Ranvier by interacting with extracellular matrix components, while NF155 mediates paranodal junction formation by binding to contactin-1/Caspr1 complexes in myelinated axons. Dysregulation of NFASC is implicated in neurological disorders like multiple sclerosis, Guillain-Barré syndrome, and autism spectrum disorders.
Recombinant NFASC proteins are engineered to study its structure-function relationships, ligand interactions, and pathological mechanisms. Typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, these proteins retain functional epitopes for binding partners like contactin-1. Caspr, and ankyrin-G. Purification often involves affinity tags (e.g., Fc-fusion or His-tag) followed by size-exclusion chromatography. Researchers use recombinant NFASC to investigate autoimmune antibody responses, develop diagnostic assays, or screen therapeutic agents targeting neuroinflammatory diseases. Its application extends to deciphering axonal regeneration pathways and myelination defects in demyelinating models. Quality validation includes SDS-PAGE, Western blot, and surface plasmon resonance (SPR) to confirm purity and binding activity.
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