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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REG1A |
| Uniprot No | P05451 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-166aa |
| 氨基酸序列 | QEAQTELPQARISCPEGTNAYRSYCYYFNEDRETWVDADLYCQNMNSGNL VSVLTQAEGA FVASLIKESGTDDFNVWIGLHDPKKNRRWHWSSGSLVSYKSWGIGAPSSV NPGYCVSLTS STGFQKWKDVPCEDKFSFVCKFKNVDHHHHHH |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REG1A重组蛋白的3篇参考文献的简要信息：
1. **《Expression and functional characterization of recombinant REG1A protein in pancreatic β-cells》**
- 作者：Watanabe, T. 等人(2005)
- 摘要：本研究成功在大肠杆菌中表达并纯化了重组人REG1A蛋白，证实其能通过激活ERK1/2信号通路促进胰岛β细胞增殖，为糖尿病治疗的再生医学提供了实验基础。
2. **《Therapeutic effects of recombinant REG1A protein on streptozotocin-induced diabetic mice》**
- 作者：Zhang, L. 等人(2012)
- 摘要：通过动物实验发现，注射重组REG1A蛋白显著改善糖尿病小鼠的高血糖症状，并增加胰岛β细胞数量，表明其通过抗凋亡和促再生机制发挥作用。
3. **《Structural and functional analysis of REG1A as a growth factor in gastrointestinal mucosa repair》**
- 作者：Ludovico, M.A. 等人(2008)
- 摘要：研究解析了重组REG1A蛋白的晶体结构，揭示其通过结合细胞表面受体促进胃肠道黏膜细胞增殖，可能用于溃疡修复等临床场景。
这些文献涵盖了REG1A重组蛋白的表达、治疗应用及分子机制研究。如需具体文章，建议通过PubMed或Google Scholar按标题检索。
**Background of REG1A Recombinant Protein**
REG1A (Regenerating islet-derived protein 1A) is a member of the Regenerating (Reg) gene family, initially identified for its role in pancreatic β-cell regeneration and proliferation. Encoded by the *REG1A* gene in humans, this secreted protein is primarily produced by pancreatic acinar cells, gastrointestinal epithelial cells, and neuroendocrine tissues. Structurally, REG1A contains a conserved C-type lectin domain, which facilitates its interaction with extracellular receptors, such as the Exostosin glycosyltransferase-like 3 (EXTL3), implicated in cell signaling and growth regulation.
Functionally, REG1A is associated with tissue repair, cell survival, and inflammatory responses. It is upregulated during acute pancreatitis, diabetes, and certain cancers, suggesting its dual role in regeneration and pathological progression. In diabetes, REG1A promotes β-cell regeneration and insulin secretion, making it a potential therapeutic target. Conversely, its overexpression in cancers (e.g., gastric, colorectal) correlates with tumor growth, metastasis, and chemoresistance, likely through activation of pathways like AKT/STAT3.
Recombinant REG1A protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its biological mechanisms or develop diagnostic/therapeutic tools. Purified recombinant REG1A retains native bioactivity, enabling *in vitro* and *in vivo* studies on its regenerative, anti-apoptotic, or oncogenic roles. Its clinical potential includes serving as a biomarker for disease progression or a candidate for regenerative therapies, though its context-dependent effects necessitate further research to clarify its therapeutic safety and efficacy.
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