| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CANT1 |
| Uniprot No | Q8WVQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 63-401aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMRPAPG RPPTHNAHNW RLGQAPANWY NDTYPLSPPQ RTPAGIRYRI AVIADLDTES RAQEENTWFS YLKKGYLTLS DSGDKVAVEW DKDHGVLESH LAEKGRGMEL SDLIVFNGKL YSVDDRTGVV YQIEGSKAVP WVILSDGDGT VEKGFKAEWL AVKDERLYVG GLGKEWTTTT GDVVNENPEW VKVVGYKGSV DHENWVSNYN ALRAAAGIQP PGYLIHESAC WSDTLQRWFF LPRRASQERY SEKDDERKGA NLLLSASPDF GDIAVSHVGA VVPTHGFSSF KFIPNTDDQI IVALKSEEDS GRVASYIMAF TLDGRFLLPE TKIGSVKYEG IEFI |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CANT1重组蛋白** 的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Recombinant human CANT1: Expression, purification, and enzymatic characterization*
**作者**：Smith A, et al.
**摘要**：本研究报道了人源CANT1重组蛋白在大肠杆菌中的高效表达及纯化方法，通过体外酶活实验证实其钙依赖性核苷酸酶活性，并分析了pH和金属离子对活性的影响。
2. **文献名称**：*CANT1 mutations alter chondrocyte differentiation via defective glycosylation in Desbuquois dysplasia*
**作者**：Chen L, et al.
**摘要**：利用重组CANT1蛋白进行功能挽救实验，证明其通过调控蛋白糖基化途径影响软骨细胞分化，揭示了CANT1基因突变导致骨骼发育异常的分子机制。
3. **文献名称**：*Structural insights into the catalytic mechanism of CANT1 by X-ray crystallography*
**作者**：Tanaka K, et al.
**摘要**：通过解析重组CANT1蛋白的晶体结构，阐明了其活性中心的构象变化及底物结合模式，为开发靶向CANT1的小分子抑制剂提供了结构基础。
4. **文献名称**：*CANT1 overexpression promotes tumor invasion via ERK signaling in pancreatic cancer*
**作者**：Wang Y, et al.
**摘要**：研究发现胰腺癌中重组CANT1蛋白的高表达通过激活ERK信号通路增强肿瘤细胞侵袭能力，提示其作为潜在治疗靶点的可能性。
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**注意**：以上文献为示例，实际研究中请通过 **PubMed、Google Scholar** 或 **Web of Science** 查询真实发表的论文。
**Background of CANT1 Recombinant Protein**
CANT1 (Calcium-Activated Nucleotidase 1) is an enzyme encoded by the *CANT1* gene, belonging to the apyrase family of hydrolytic enzymes. It catalyzes the cleavage of nucleoside diphosphates (e.g., UDP, GDP) and triphosphates, relying on calcium ions for activation. CANT1 is primarily localized to the extracellular matrix and Golgi apparatus, where it plays a role in nucleotide metabolism, potentially influencing cellular processes such as glycosylation, purinergic signaling, and cartilage development.
Mutations in the *CANT1* gene are linked to genetic disorders like Desbuquois dysplasia type 2 and severe bone dysplasia, characterized by skeletal abnormalities and joint laxity. These conditions highlight CANT1's critical role in skeletal growth and extracellular matrix homeostasis. Dysregulated CANT1 expression has also been observed in cancers, suggesting its involvement in tumor progression.
Recombinant CANT1 protein is engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to produce a purified, bioactive form of the enzyme. This tool enables researchers to study CANT1's enzymatic activity, substrate specificity, and interactions with other biomolecules. It is widely used in *in vitro* assays to investigate disease mechanisms, screen potential inhibitors, or explore therapeutic strategies targeting CANT1-related pathologies.
Additionally, recombinant CANT1 serves as an antigen for antibody development, aiding in diagnostic applications or tissue-specific expression studies. Its structural analysis (e.g., X-ray crystallography) has provided insights into calcium-binding domains and catalytic sites, guiding drug design efforts. Ongoing research aims to clarify CANT1's broader physiological roles and its potential as a biomarker or therapeutic target in bone disorders and cancer.
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