| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPC5 |
| Uniprot No | P78333 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-572aa |
| 氨基酸序列 | MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGAVRGLPDSPRAGPDLQVCISKKPTCCTRKMEERYQIAARQDMQQFLQTSSSTLKFLISRNAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAASVQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQRVMGQMGRSLLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLRLYRSFYGGLADQLCANELAAADGLPCWNGEDIVKSYTQRVVGNGIKAQSGNPEVKVKGIDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGMVEQVSGDCDDEDGCGGSGSGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVATESMTFTLISVVMLLPGIW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPC5重组蛋白的虚构参考文献示例(注：实际文献需通过学术数据库查询)：
---
1. **文献名称**：*Expression and Purification of Recombinant GPC5 in Mammalian Cells for Structural Analysis*
**作者**：Wang Y. et al.
**摘要**：本研究报道了在HEK293细胞中高效表达可溶性GPC5重组蛋白的方法，通过亲和层析和尺寸排阻色谱纯化，并利用圆二色光谱分析其二级结构，为后续功能研究提供了基础。
2. **文献名称**：*GPC5 Recombinant Protein Enhances Wnt/β-Catenin Signaling in Lung Cancer Cells*
**作者**：Li J. et al.
**摘要**：研究发现，外源性重组GPC5蛋白通过结合Wnt配体，激活β-catenin信号通路，促进肺癌细胞增殖和侵袭，提示GPC5可能作为肿瘤治疗的潜在靶点。
3. **文献名称**：*Development of a GPC5-Based Recombinant Protein for Targeted Drug Delivery in Glioblastoma*
**作者**：Zhang L. et al.
**摘要**：通过融合GPC5重组蛋白与纳米载体，构建了靶向胶质母细胞瘤的药物递送系统，体外实验显示其能特异性结合肿瘤细胞并增强化疗药物疗效。
4. **文献名称**：*Functional Characterization of GPC5 in Hedgehog Signaling Using Recombinant Protein*
**作者**：Kim S. et al.
**摘要**：利用重组GPC5蛋白验证其作为Hedgehog信号共受体的作用，发现其通过硫酸乙酰肝素链介导配体-受体相互作用，调控胚胎发育过程中的细胞分化。
---
建议通过PubMed、Google Scholar等平台以“GPC5 recombinant protein”为关键词检索真实文献。
**Background of GPC5 Recombinant Protein**
Glypican-5 (GPC5) is a member of the glypican family, a group of heparan sulfate proteoglycans anchored to the cell membrane via glycosylphosphatidylinositol (GPI). These proteins play critical roles in modulating signaling pathways by interacting with growth factors, morphogens, and receptors. GPC5. encoded by the *GPC5* gene, is involved in regulating cellular processes such as proliferation, differentiation, and apoptosis, primarily through interactions with Wnt, FGF, and BMP signaling pathways. Dysregulation of GPC5 has been implicated in various diseases, including cancers, neurological disorders, and developmental anomalies.
Recombinant GPC5 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, bioactive form of the protein. This allows researchers to study its structure-function relationships, ligand-binding properties, and therapeutic potential. In cancer biology, GPC5 is of particular interest due to its dual role as a tumor suppressor or promoter, depending on context. For instance, reduced GPC5 expression is linked to poor prognosis in lung adenocarcinoma, while overexpression is observed in certain gliomas.
The recombinant protein often includes specific domains (e.g., the core protein without GPI anchor) and tags (e.g., His-tag or Fc-fusion) for purification and detection. Applications range from *in vitro* assays exploring signaling mechanisms to preclinical studies evaluating GPC5-targeted therapies. Additionally, GPC5 recombinant protein serves as a tool for antibody development and biomarker discovery, highlighting its versatility in both basic research and translational medicine.
Overall, GPC5 recombinant protein provides a valuable resource for dissecting its biological roles and advancing therapeutic strategies for GPC5-associated diseases.
×
