| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | MDGA2 |
| Uniprot No | P60755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-949aa |
| 氨基酸序列 | MDLVYGLVWLLTVLLEGISGQGVYAPPTVRIVHSGLACNIEEERYSERVYTIREGETLELTCLVTGHPRPQIRWTKTAGSASDRFQDSSVFNETLRITNIQRHQGGRYYCKAENGLGSPAIKSIRVDVYYLDDPVVTVHQSIGEAKEQFYYERTVFLRCVANSNPPVRYSWRRGQEVLLQGSDKGVEIYEPFFTQGETKILKLKNLRPQDYANYSCIASVRNVCNIPDKMVSFRLSNKTASPSIKLLVDDPIVVNPGEAITLVCVTTGGEPTPSLTWVRSFGTLPEKIVLNGGTLTIPAITSDDAGTYSCIANNNVGNPAKKSTNIIVRALKKGRFWITPDPYHKDDNIQIGREVKISCQVEAVPSEELTFSWFKNGRPLRSSERMVITQTDPDVSPGTTNLDIIDLKFTDFGTYTCVASLKGGGISDISIDVNISSSTVPPNLTVPQEKSPLVTREGDTIELQCQVTGKPKPIILWSRADKEVAMPDGTMQMESYDGTLRIVNVSREMSGMYRCQTSQYNGFNVKPREALVQLIVQYPPAVEPAFLEIRQGQDRSVTMSCRVLRAYPIRVLTYEWRLGNKLLRTGQFDSQEYTEYPLKSLSNENYGVYNCSIINEAGAGRCSFLVTGKAYAPEFYYDTYNPVWQNRHRVYSYSLQWTQMNPDAVDRIVAYRLGIRQAGQQRWWEQEIKINGNIQKGELITYNLTELIKPEAYEVRLTPLTKFGEGDSTIRVIKYTGEFHCGFEDGNICLFTQDDTDNFDWTKQSTATRNTKYTPNTGPSADRSGSKEGFYMYIETSRPRLEGEKARLLSPVFSIAPKNPYGPTNSAYCFSFFYHMYGQHIGVLNVYLRLKGQTTIENPLWSSSGNKGQRWNEAHVNIYPITSFQLIFEGIRGPGIEGDIAIDDVSIAEGECAKQDLPTKNSVDGAVGILVHIWLFPVIILISILSPRR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MDGA2重组蛋白相关的文献摘要(模拟示例，供参考)：
1. **文献名称**: "MDGA2 acts as a synaptogenic adhesion molecule by binding to neuroligin-2"
**作者**: Kim JA, et al.
**摘要**: 本研究通过重组MDGA2蛋白实验，发现其通过特异性结合突触后神经配蛋白neuroligin-2.抑制GABA能突触的形成，揭示了MDGA2在抑制性突触调控中的作用机制。
2. **文献名称**: "Structural basis of MDGA2-mediated synaptic differentiation"
**作者**: Gangwar SP, et al.
**摘要**: 利用重组MDGA2蛋白的晶体结构解析，揭示了其MAM结构域与神经配蛋白相互作用的分子基础，为理解MDGA2在突触分化和神经发育障碍中的功能提供结构依据。
3. **文献名称**: "MDGA2 negatively regulates hippocampal synaptic plasticity and learning"
**作者**: Connor SA, et al.
**摘要**: 通过体外重组蛋白实验结合小鼠模型，证明MDGA2通过调控NMDA受体功能影响海马突触可塑性和空间学习能力，提示其与认知障碍疾病的潜在关联。
注：以上内容为模拟文献摘要，实际文献需通过PubMed或Google Scholar检索关键词 "MDGA2 recombinant protein" 获取。部分真实相关研究可参考Ann Marie Craig、Thomas Südhof等团队发表的论文。
MDGA2 (Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2) is a neuronal cell adhesion molecule implicated in regulating synaptic development and function. It belongs to the MDGA protein family, characterized by conserved immunoglobulin (Ig)-like, fibronectin type III (FN3), and pseudokinase domains. MDGA2 is primarily expressed in the brain, where it interacts with postsynaptic proteins such as neuroligins and plays a role in modulating excitatory and inhibitory synapse formation. Studies suggest it acts as a selective inhibitor of neuroligin-1-mediated excitatory synapse development by sterically hindering neuroligin-1/neurexin interactions, thereby influencing neural circuit balance.
Research has linked MDGA2 to neurodevelopmental disorders. Genetic variants or altered expression of MDGA2 are associated with autism spectrum disorders (ASD), schizophrenia, and intellectual disability, highlighting its importance in cognitive function. Its role in GABAergic synapse regulation further connects it to epilepsy and hyperexcitability conditions. Structural studies reveal that MDGA2's Ig and FN3 domains mediate protein-protein interactions, while its pseudokinase domain may have regulatory functions despite lacking catalytic activity.
Recombinant MDGA2 proteins are engineered for in vitro studies to dissect its molecular mechanisms. These proteins are typically produced in mammalian (e.g., HEK293) or insect cell systems to ensure proper post-translational modifications. Purification often involves affinity tags (e.g., His-tag or Fc-fusion) followed by chromatography. Recombinant MDGA2 enables biochemical assays, structural analysis (e.g., crystallography or cryo-EM), and screening for therapeutic compounds targeting MDGA2-associated pathways. Its application extends to disease modeling, antibody development, and exploring synaptic plasticity mechanisms, offering potential insights into neuropathology and treatment strategies.
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