| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLAMF5/CD84 |
| Uniprot No | Q9UIB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-225aa |
| 氨基酸序列 | KDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETA PVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTT KRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPL GEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFRT HHTG |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLAMF5/CD84重组蛋白的3篇参考文献示例：
1. **文献名称**：*Structural and functional characterization of recombinant SLAMF5/CD84 reveals its role in immune synapse formation*
**作者**：M. Garcia et al.
**摘要**：本研究通过昆虫细胞系统表达并纯化了重组SLAMF5胞外域蛋白，结合X射线晶体学解析了其三维结构，发现其通过同源二聚化介导免疫细胞间黏附，并促进T细胞与抗原呈递细胞的突触形成。
2. **文献名称**：*SLAMF5/CD84 modulates B-cell signaling and survival via interaction with SAP adaptor protein*
**作者**：R. Kumar et al.
**摘要**：通过重组SLAMF5蛋白与SAP(SLAM-associated protein)的体外结合实验，证明SLAMF5胞内段依赖SAP传递下游信号，调控B细胞存活及抗体分泌，为靶向SLAMF5治疗B细胞淋巴瘤提供依据。
3. **文献名称**：*CD84-mediated homophilic interaction enhances myeloma cell adhesion and drug resistance*
**作者**：L. Chen et al.
**摘要**：利用重组CD84蛋白阻断实验发现，多发性骨髓瘤细胞通过CD84同源结合增强与微环境的黏附，激活PI3K/Akt通路，进而促进化疗耐药，提示CD84作为潜在治疗靶点。
(注：以上文献为示例，实际引用需根据具体研究内容检索真实数据库。)
SLAMF5 (Signaling Lymphocytic Activation Molecule Family member 5), also known as CD84. is a cell surface glycoprotein belonging to the immunoglobulin (Ig) superfamily. It functions as a self-ligand adhesion receptor involved in modulating immune cell interactions, particularly within the hematopoietic system. Structurally, it contains an extracellular region with two Ig-like domains (V and C2-type), a transmembrane domain, and a cytoplasmic tail with conserved tyrosine-based signaling motifs that recruit adaptor proteins like SAP (SLAM-associated protein) and EAT-2. These interactions enable SLAMF5/CD84 to regulate bidirectional signaling pathways influencing cell activation, differentiation, and immune homeostasis.
As a member of the SLAM family, CD84 is predominantly expressed on B cells, T cells, dendritic cells, platelets, and macrophages. It mediates homophilic interactions (binding to CD84 on adjacent cells) to facilitate immune synapse formation, cell adhesion, and intercellular communication. Its role extends to both innate and adaptive immunity, including modulating B-cell receptor signaling, platelet activation, and cytokine production. Dysregulation of CD84 has been implicated in autoimmune disorders (e.g., lupus) and hematological malignancies, making it a potential therapeutic target.
Recombinant SLAMF5/CD84 proteins are engineered to include specific functional domains, often the extracellular region, for in vitro studies. Produced via mammalian expression systems (e.g., HEK293 or CHO cells), these proteins retain binding capacity and are utilized to investigate receptor-ligand interactions, signal transduction mechanisms, and competitive inhibition assays. They serve as critical tools in developing antibody-based therapies, high-throughput screening for drug discovery, and structural studies to map binding epitopes. Additionally, tagged versions (e.g., Fc or His-tags) enable applications in flow cytometry, ELISA, and protein-protein interaction analyses, advancing research in immunology and cancer biology.
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