| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN11 |
| Uniprot No | Q06124 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-593aa |
| 氨基酸序列 | TSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR |
| 预测分子量 | 72.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTPN11/SHP-2重组蛋白的参考文献及其摘要概括：
1. **文献名称**：*Structural and Pathogenic Diversity of Shp2 Mutants*
**作者**：Tartaglia M, et al.
**摘要**：解析PTPN11编码的SHP-2磷酸酶在努南综合征和白血病中的突变热点(如E76K)，通过重组蛋白实验揭示突变体导致组成型激活的分子机制，及其对RAS-MAPK信号通路的异常调控。
2. **文献名称**：*Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by receptor tyrosine kinases*
**作者**：Chen YN, et al.
**摘要**：报道一种新型SHP-2变构抑制剂(SHP099)，通过重组SHP-2蛋白的晶体结构分析，阐明其结合位点及抑制机制，并在体内外实验中验证其对RTK驱动的肿瘤生长的抑制作用。
3. **文献名称**：*Mechanism of activating mutations and allostery in SHP2 phosphatase*
**作者**：LaRochelle JR, et al.
**摘要**：利用重组SHP-2突变体(如D61G、E76K)，结合X射线晶体学和酶动力学，揭示致癌突变通过破坏自抑制构象增强催化活性，并提出变构调控模型。
4. **文献名称**：*Expression and purification of active SHP-2 protein tyrosine phosphatase in E. coli*
**作者**：Keilhack H, et al.
**摘要**：描述在大肠杆菌中高效表达可溶性重组SHP-2蛋白的方法，优化纯化流程并验证其磷酸酶活性，为后续功能研究提供可靠蛋白来源。
以上文献涵盖PTPN11重组蛋白的结构解析、突变致病机制、药物开发及表达技术等领域。
**Background of PTPN11 Recombinant Protein**
The PTPN11 gene encodes SHP-2 (Src homology region 2-containing protein tyrosine phosphatase-2), a ubiquitously expressed non-receptor protein tyrosine phosphatase. SHP-2 plays a critical role in regulating intracellular signaling pathways, particularly the RAS/mitogen-activated protein kinase (RAS/MAPK) cascade, which governs cell proliferation, differentiation, survival, and metabolism. Structurally, SHP-2 contains two N-terminal Src homology 2 (SH2) domains that mediate protein interactions and a C-terminal catalytic phosphatase domain. Unlike most phosphatases, SHP-2 requires autoinhibitory conformational changes to activate its enzymatic activity, making its regulation tightly controlled.
Mutations in PTPN11 are strongly linked to human diseases. Gain-of-function mutations are associated with Noonan syndrome, LEOPARD syndrome, and certain cancers (e.g., juvenile myelomonocytic leukemia, acute myeloid leukemia), while loss-of-function variants may contribute to metabolic disorders. These mutations often alter SHP-2’s phosphatase activity or interaction capabilities, leading to dysregulated signaling.
Recombinant PTPN11 proteins are engineered to study SHP-2’s biochemical properties, structure-function relationships, and disease mechanisms. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins are purified with tags (e.g., His-tag) for ease of isolation. They serve as essential tools for *in vitro* assays, including enzymatic activity profiling, inhibitor screening, and interaction studies with substrates like GRB2 or IRS1. Additionally, recombinant mutants mimicking pathogenic variants help elucidate how specific amino acid changes drive signaling abnormalities.
In drug discovery, PTPN11 recombinant proteins aid in developing targeted therapies, especially for cancers with SHP-2 hyperactivity. Their use also extends to diagnostic applications, such as antibody validation or serving as positive controls in Western blotting. Overall, these recombinant tools are vital for advancing both basic research and translational studies of SHP-2-related pathologies.
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