| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLEC4M |
| Uniprot No | Q9H2X3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 78-399aa |
| 氨基酸序列 | SLSQEQSEQD AIYQNLTQLK AAVGELSEKS KLQEIYQELT QLKAAVGELP EKSKLQEIYQ ELTRLKAAVG ELPEKSKLQE IYQELTRLKA AVGELPEKSK LQEIYQELTR LKAAVGELPE KSKLQEIYQE LTELKAAVGE LPEKSKLQEI YQELTQLKAA VGELPDQSKQ QQIYQELTDL KTAFERLCRH CPKDWTFFQG NCYFMSNSQR NWHDSVTACQ EVRAQLVVIK TAEEQNFLQL QTSRSNRFSW MGLSDLNQEG TWQWVDGSPL SPSFQRYWNS GEPNNSGNED CAEFSGSGWN DNRCDVDNYW ICKKPAACFR DE |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLEC4M重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Structural characterization of recombinant CLEC4M: Insights into its carbohydrate recognition domain"*
**作者**：Drickamer K., et al.
**摘要**：本研究通过重组表达和纯化CLEC4M的胞外结构域，结合X射线晶体学解析了其碳水化合物识别域(CRD)的三维结构，揭示了其与甘露糖和岩藻糖特异性结合的分子机制。
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2. **文献名称**：*"CLEC4M functions as a receptor for hepatitis C virus glycoprotein E2"*
**作者**：Lozach P.Y., et al.
**摘要**：通过体外重组CLEC4M蛋白实验，证明其能够直接结合丙型肝炎病毒(HCV)的E2糖蛋白，并促进病毒进入宿主细胞，提示CLEC4M在HCV感染中可能作为辅助受体发挥作用。
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3. **文献名称**：*"Polymorphism in CLEC4M modulates N-glycosylation and impacts HIV-1 transmission"*
**作者**：Barreiro L.B., et al.
**摘要**：研究利用重组CLEC4M蛋白变异体，发现其基因多态性导致N-糖基化模式差异，进而影响与HIV-1 gp120的结合能力，为解释人群间艾滋病易感性差异提供了分子基础。
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(注：以上文献信息为示例性概括，实际引用需以具体发表内容为准。)
**Background of CLEC4M Recombinant Protein**
CLEC4M (C-type lectin domain family 4 member M), also known as DC-SIGNR (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin-related) or CD299. is a transmembrane protein belonging to the C-type lectin superfamily. It is primarily expressed on endothelial cells in liver sinusoids, lymph nodes, and placental tissue, as well as on certain immune cells. Structurally, CLEC4M contains a carbohydrate-recognition domain (CRD) that enables binding to high-mannose and fucosylated glycans on pathogens, facilitating pathogen recognition and immune modulation.
CLEC4M plays a dual role in immunity. It acts as a pathogen receptor, interacting with viruses (e.g., HIV, HCV, SARS-CoV-2), bacteria, and fungi to mediate their internalization or promote immune cell interactions. Conversely, it may dampen excessive inflammation by modulating Toll-like receptor (TLR) signaling. Its ability to bind viral envelope glycoproteins also positions it as a potential entry co-receptor, influencing viral tropism and infection efficiency.
Recombinant CLEC4M protein is engineered for research and therapeutic applications. Produced via expression systems like mammalian cells or *E. coli*, it retains the CRD’s glycan-binding activity. This protein is widely used to study host-pathogen interactions, screen antiviral compounds, and develop glycan-based inhibitors. Additionally, CLEC4M’s genetic polymorphisms, linked to altered ligand affinity and disease susceptibility (e.g., HIV progression, preeclampsia), make it a focus in personalized medicine research.
In summary, CLEC4M recombinant protein serves as a critical tool for dissecting immune mechanisms, advancing antiviral strategies, and exploring its therapeutic potential in infectious and inflammatory diseases.
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