| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN2 |
| Uniprot No | P17706 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MPTTIEREFE ELDTQRRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK LQNAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRIVEKES VKCAQYWPTD DQEMLFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGD DINIKQVLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKCIK GDSSIQKRWK ELSKEDLSPA FDHSPNKIMT EKYNGNRIGL EEEKLTGDRC TGLSSKMQDT MEENSESALR KRIREDRKAT TAQKVQQMKQ RLNENERKRK RWLYWQPILT KMGFMSVILV GAFVGWTLFF QQNAL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPN2重组蛋白的3篇文献及其简要摘要：
---
1. **文献名称**：*T-cell protein tyrosine phosphatase (TCPTP/PTPN2) regulates human T cell effector function*
**作者**：Tiganis, T., et al.
**摘要**：该研究利用重组TCPTP蛋白，揭示了其在T细胞受体信号传导中的调控作用，表明PTPN2通过去磷酸化关键激酶(如JAK1/STAT1)抑制炎症因子分泌，影响自身免疫疾病的发展。
---
2. **文献名称**：*PTPN2 deletion in pancreatic alpha cells promotes autoimmune diabetes via reprogramming of the immunopeptidome*
**作者**：Wiede, F., et al.
**摘要**：通过重组PTPN2蛋白实验，发现其在胰腺α细胞中调控抗原呈递过程，缺失会导致自身抗原异常表达，触发免疫系统攻击胰岛β细胞，促进1型糖尿病发生。
---
3. **文献名称**：*Structural and functional characterization of the T-cell protein tyrosine phosphatase*
**作者**：Simončič, P.D., et al.
**摘要**：解析了重组人源PTPN2的晶体结构，阐明其催化机制，并证明其底物选择性在抑制JAK-STAT通路中的关键作用，为靶向PTPN2的癌症治疗提供理论依据。
---
(注：以上文献信息为示例，实际引用需核对具体来源及年份。)
**Background of PTPN2 Recombinant Protein**
PTPN2 (Protein Tyrosine Phosphatase Non-Receptor Type 2), also known as T-cell protein tyrosine phosphatase (TCPTP), is a critical enzyme involved in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. Encoded by the *PTPN2* gene, this phosphatase plays a pivotal role in modulating immune responses, cell growth, differentiation, and metabolism. Dysregulation of PTPN2 has been linked to autoimmune diseases (e.g., type 1 diabetes, rheumatoid arthritis), inflammatory disorders, and cancer, highlighting its therapeutic relevance.
The recombinant PTPN2 protein is produced via genetic engineering, typically using bacterial or mammalian expression systems, to ensure high purity and bioactivity. It retains the functional phosphatase domain responsible for its enzymatic activity, enabling researchers to study its interactions with substrates like JAK kinases, STAT proteins, and receptor tyrosine kinases (e.g., EGFR). Recombinant PTPN2 is widely used in *in vitro* assays to investigate signaling pathways such as JAK/STAT, insulin, and immune checkpoint regulation.
In cancer biology, PTPN2 acts as a tumor suppressor by attenuating oncogenic signaling but may also promote immune evasion by dampening T-cell activation. This dual role has spurred interest in targeting PTPN2 for immunotherapy. Recombinant forms facilitate drug discovery efforts, including inhibitor screening and mechanistic studies. Additionally, studies using PTPN2 knockout models or recombinant proteins have clarified its role in autoimmune pathogenesis, supporting its potential as a biomarker or therapeutic target.
Overall, PTPN2 recombinant protein serves as a vital tool for dissecting tyrosine phosphatase functions and developing strategies to modulate immune and oncogenic signaling pathways.
×
