| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAT1 |
| Uniprot No | P42224-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-712aa |
| 氨基酸序列 | MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK RRQQSACIGG PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK LQELNYNLKV KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL AAEFRHLQLK EQKNAGTRTN EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVAE PRNLSFFLTP PCARWAQLSE VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT RFCKENINDK NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG YIKTELISVS EV |
| 预测分子量 | 111 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAT1重组蛋白的3篇代表性文献示例(注：文献信息为模拟，建议通过学术数据库核实)：
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1. **文献名称**：*"Production of Recombinant STAT1 in Escherichia coli for Functional Studies"*
**作者**：Chen, X.,等人
**摘要**：该研究描述了一种在大肠杆菌中高效表达并纯化重组人STAT1蛋白的方法。通过His标签亲和层析获得高纯度蛋白，并验证其与干扰素响应元件(ISRE)的DNA结合能力，证实重组蛋白在体外磷酸化后具有转录激活功能。
2. **文献名称**：*"Crystal Structure of Phosphorylated STAT1 Dimer Bound to DNA"*
**作者**：Becker, S.,等人
**摘要**：本文首次解析了磷酸化STAT1二聚体的晶体结构，揭示了其通过SH2结构域介导的二聚化机制及与靶DNA的结合模式，为理解STAT1在干扰素信号中的转录调控提供了结构基础。
3. **文献名称**：*"Functional Reconstitution of the JAK-STAT Pathway Using Recombinant Proteins"*
**作者**：Darnell, J.E.,等人
**摘要**：研究利用重组STAT1、JAK激酶和干扰素受体构建了体外信号通路模型，证明STAT1的磷酸化、核转位及DNA结合活性，确立了其在JAK-STAT通路中的核心作用。
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**建议**：可通过PubMed或Google Scholar搜索关键词“STAT1 recombinant protein expression/purification/structure”获取最新文献。真实文献可能包括《Nature》《Cell》或《JBC》等期刊中的研究。
**Background of STAT1 Recombinant Protein**
STAT1 (Signal Transducer and Activator of Transcription 1) is a critical transcription factor involved in mediating cellular responses to cytokines, particularly interferons (IFNs). As a member of the STAT protein family, it plays a central role in the Janus kinase (JAK)-STAT signaling pathway, which regulates immune defense, cell proliferation, apoptosis, and differentiation. STAT1 exists in two isoforms, STAT1α (91 kDa) and STAT1β (84 kDa), generated by alternative splicing. Upon activation by cytokines or growth factors, STAT1 is phosphorylated, dimerizes, and translocates to the nucleus to bind specific DNA sequences, initiating transcription of target genes implicated in antiviral responses, antitumor immunity, and inflammation.
Recombinant STAT1 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells*) to produce functional, purified STAT1 for research and therapeutic applications. Its recombinant form retains key structural domains, including an N-terminal domain for oligomerization, a coiled-coil domain for protein interactions, a DNA-binding domain, and a C-terminal transactivation domain. Researchers utilize STAT1 recombinant protein to study signaling mechanisms, screen inhibitors, or investigate dysregulated pathways in diseases like cancer, autoimmune disorders, or viral infections.
Studies have highlighted STAT1's dual role: while its activation promotes tumor suppression and pathogen clearance, chronic activation may drive immune dysfunction or resistance to therapies. Recombinant STAT1 enables in vitro assays (e.g., phosphorylation, DNA-binding studies) to dissect these complex roles. Additionally, it serves as a tool for developing targeted therapies, such as JAK-STAT inhibitors, to modulate immune responses in conditions like rheumatoid arthritis or IFN-related diseases.
Overall, STAT1 recombinant protein is a vital resource for advancing both basic research and translational medicine in immunology and oncology.
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