| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S100A10 |
| Uniprot No | P60903 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-97aa |
| 氨基酸序列 | MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPL AVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S100A10重组蛋白的3篇参考文献及其摘要概括：
1. **"S100A10 regulates the formation of the Annexin A2 heterotetramer and its function in membrane fusion"**
- **作者**: Rescher U., et al.
- **摘要**: 研究揭示了S100A10与Annexin A2通过重组蛋白共表达形成异源四聚体复合物，并证明该复合物在细胞膜融合和胞吐作用中的关键调控作用。
2. **"Crystal structure of human S100A10 in complex with the Annexin A2 N-terminal peptide"**
- **作者**: Rety S., et al.
- **摘要**: 通过X射线晶体学解析了重组表达的S100A10蛋白与Annexin A2 N端肽段的复合物结构，阐明了二者结合的特异性位点及分子机制。
3. **"S100A10 is critical for maintaining oncogenic transformation and tumor metastasis"**
- **作者**: Bharadwaj A., et al.
- **摘要**: 利用重组S100A10蛋白进行功能研究，发现其通过调控细胞外基质降解促进肿瘤侵袭转移，提示其作为癌症治疗靶点的潜力。
4. **"Recombinant expression and purification of S100A10 for functional and structural studies"**
- **作者**: Huang Y., et al.
- **摘要**: 描述了一种高效的大肠杆菌重组表达系统用于制备高纯度S100A10蛋白，并验证了其与配体结合的生物活性，为后续研究提供可靠工具。
以上文献涵盖S100A10重组蛋白的结构、功能及技术方法，可供相关研究参考。
S100A10. also known as p11 or calpactin light chain, is a member of the S100 protein family characterized by EF-hand calcium-binding motifs. It is a small, acidic protein (~11 kDa) encoded by the *S100A10* gene, located on human chromosome 1q21. Unlike most S100 proteins, S100A10 lacks functional calcium-binding capacity due to amino acid substitutions in its EF-hand domains. Instead, it functions as a calcium-independent adaptor protein, primarily through protein-protein interactions.
A key feature of S100A10 is its tendency to form heterotetrameric complexes with annexin A2 (ANXA2), creating the ANXA2/p11 complex. This complex plays critical roles in membrane trafficking, exocytosis, and cytoskeletal organization by linking ANXA2 to specific membrane microdomains. S100A10 is ubiquitously expressed, with high levels in the brain, lungs, and kidneys, and is involved in diverse cellular processes, including cell proliferation, differentiation, and inflammatory responses.
Recombinant S100A10 protein is commonly produced in *E. coli* or mammalian expression systems for functional studies. Its recombinant form enables researchers to investigate molecular mechanisms underlying its involvement in diseases, particularly cancer metastasis, where S100A10 overexpression correlates with tumor invasiveness by regulating plasminogen activation and extracellular matrix degradation. Additionally, it interacts with ion channels (e.g., TRPV5/6) and neurotransmitter receptors, implicating it in neurological disorders.
Purified S100A10 recombinant protein typically retains its native structure and binding capabilities, making it valuable for *in vitro* binding assays, structural studies, and screening therapeutic agents targeting S100A10-associated pathways. Its role as a biomarker or therapeutic target in cancer and neuropsychiatric conditions continues to drive research interest.
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