| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP3 |
| Uniprot No | P51452 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSGSFELSVQDLNDLLSDGSGCYSLPSQPC NEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHNTNANFYKDS GITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRS PTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEG KLKP |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP3重组蛋白的3篇代表性文献(注：以下内容为示例，实际文献需通过数据库查询)：
1. **文献名称**: "DUSP3 regulates the oncogenic potential of ERK in human cancer cells"
**作者**: Alonso A, et al.
**摘要**: 研究通过重组DUSP3蛋白揭示其通过去磷酸化ERK1/2抑制癌细胞增殖，表明DUSP3作为肿瘤抑制因子的潜在作用。
2. **文献名称**: "Structural basis for the substrate specificity of DUSP3 phosphatase"
**作者**: Chen Y, et al.
**摘要**: 利用重组DUSP3蛋白进行晶体结构分析，阐明其对MAP激酶家族(如ERK和p38)的特异性识别机制。
3. **文献名称**: "DUSP3 modulates innate immune responses by regulating TLR4-induced NF-κB activation"
**作者**: Jeffrey KL, et al.
**摘要**: 通过体外重组蛋白实验，证明DUSP3通过去磷酸化TAK1负调控TLR4信号通路，影响巨噬细胞炎症因子释放。
如需具体文献，建议在PubMed或Web of Science中检索关键词“DUSP3 recombinant protein”。
Dual specificity phosphatase 3 (DUSP3), also known as vaccinia H1-related phosphatase (VHR), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. Unlike classical tyrosine or serine/threonine phosphatases, DUSP3 can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues on target proteins. It is a relatively small protein (~21 kDa) encoded by the DUSP3 gene in humans, characterized by a conserved catalytic domain with a signature HCXXGXXR motif essential for its phosphatase activity.
DUSP3 primarily modulates the ERK, JNK, and p38 MAPK pathways by dephosphorylating activated MAPKs, thereby influencing cellular processes such as proliferation, differentiation, and apoptosis. Its activity is tightly regulated by post-translational modifications, including phosphorylation and oxidative inactivation. Studies have linked DUSP3 to diverse physiological and pathological roles, including immune response regulation, cell cycle control, and cancer progression. For instance, it exhibits context-dependent oncogenic or tumor-suppressive functions in cancers like breast, lung, and cervical malignancies.
Recombinant DUSP3 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional and structural studies. Purified recombinant DUSP3 enables in vitro analysis of its enzymatic kinetics, substrate specificity, and interactions with signaling partners. Researchers utilize it to explore mechanisms of MAPK pathway regulation, screen potential inhibitors for therapeutic development, or investigate its role in diseases. Recent work also highlights its involvement in vascular homeostasis and DNA damage response, expanding its biomedical relevance. Despite its well-studied phosphatase activity, ongoing research aims to clarify its tissue-specific functions and therapeutic targeting potential in inflammation and cancer.
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