| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLRT1 |
| Uniprot No | Q9NZU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-524aa |
| 氨基酸序列 | IDSTTCPSVCRCDNGFIYCNDRGLTSIPADIPDDATTLYLQNNQINNAGI PQDLKTKVNVQVIYLYENDLDEFPINLPRSLRELHLQDNNVRTIARDSLA RIPLLEKLHLDDNSVSTVSIEEDAFADSKQLKLLFLSRNHLSSIPSGLPH TLEELRLDDNRISTIPLHAFKGLNSLRRLVLDGNLLANQRIADDTFSRLQ NLTELSLVRNSLAAPPLNLPSAHLQKLYLQDNAISHIPYNTLAKMRELER LDLSNNNLTTLPRGLFDDLGNLAQLLLRNNPWFCGCNLMWLRDWVKARAA VVNVRGLMCQGPEKVRGMAIKDITSEMDECFETGPQGGVANAAAKTTASN HASATTPQGSLFTLKAKRPGLRLPDSNIDYPMATGDGAKTLAIHVKALTA DSIRITWKATLPASSFRLSWLRLGHSPAVGSITETLVQGDKTEYLLTALE PKSTYIICMVTMETSNAYVADETPVCAKAETADSYGPTTTLNQEQNAGPM ASLP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLRT1重组蛋白的模拟参考文献示例(内容为虚构，仅供参考)：
1. **《FLRT1重组蛋白的结构解析及其与Unc5受体的相互作用》**
作者：Smith A, et al.
摘要：本研究通过X射线晶体学解析了FLRT1胞外域重组蛋白的三维结构，发现其富含亮氨酸重复序列的结构域介导与Unc5神经导向受体的特异性结合，为理解FLRT1在轴突导向中的功能提供结构基础。
2. **《FLRT1重组蛋白在TGF-β信号通路中的调控作用》**
作者：Zhang L, et al.
摘要：利用哺乳动物细胞表达系统制备功能性FLRT1重组蛋白，证实其通过竞争性结合BMP配体抑制TGF-β信号通路活性，揭示了FLRT1在胚胎发育中的双重调控机制。
3. **《重组FLRT1蛋白促进血管内皮细胞迁移的机制研究》**
作者：Tanaka K, et al.
摘要：通过原核表达纯化获得高纯度FLRT1重组蛋白，体外实验证明其通过激活Integrin β1信号通路增强内皮细胞迁移能力，提示其在血管生成中的潜在应用价值。
4. **《FLRT1重组蛋白在胶质瘤侵袭性中的作用》**
作者：Wang X, et al.
摘要：采用His标签纯化的FLRT1重组蛋白进行功能研究，发现其通过上调MMP-2表达增强胶质瘤细胞侵袭能力，为肿瘤靶向治疗提供了新靶点。
FLRT1 (Fibronectin Leucine-Rich Transmembrane protein 1) is a member of the FLRT family, a group of transmembrane proteins characterized by leucine-rich repeats (LRRs) and a fibronectin type III (FNIII) domain. These structural features enable FLRT1 to participate in cell-cell and cell-matrix interactions, influencing adhesion and signaling processes. Initially identified for its role in developmental biology, FLRT1 is widely expressed in the nervous system, where it regulates neurite outgrowth, neuronal migration, and synaptic organization. It interacts with multiple receptors, including Unc5 receptors and latrophilins (LPHNs), modulating pathways such as Netrin signaling and planar cell polarity. Beyond neurodevelopment, FLRT1 contributes to vascular development, tissue morphogenesis, and cancer progression, where its dysregulation is linked to metastasis and angiogenesis.
Recombinant FLRT1 protein, produced via expression systems like mammalian cells or *E. coli*, retains functional domains critical for studying its biological activities. Researchers employ it to dissect molecular mechanisms, map interaction partners (e.g., with Robo receptors or integrins), and explore its dual role as both an adhesive and repulsive cue. Structural studies using recombinant FLRT1 have revealed conformational flexibility essential for ligand binding. Additionally, it serves as a tool for drug discovery, enabling high-throughput screens for modulators of FLRT1-mediated pathways. Its therapeutic potential is being explored in neurological disorders and cancer, though challenges remain in targeting its context-dependent functions. Overall, recombinant FLRT1 is a versatile reagent for unraveling its multifaceted roles in health and disease.
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