| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROR1 |
| Uniprot No | Q01973 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 429-937aa |
| 氨基酸序列 | RNNQKSSSAPVQRQPKHVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEE LGECAFGKIYKGHLYLPGMDHAQLVAIKTLKDYNNPQQWTEFQQEASLMA ELHHPNIVCLLGAVTQEQPVCMLFEYINQGDLHEFLIMRSPHSDVGCSSD EDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLH VKISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSF GVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCSEDCPPRMYSLMTEC WNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQTTSLSASPV SNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPINGYPIPPGY AAFPAAHYQPTGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQ EANIPLLPHMSIPNHPGGMGITVFGNKSQKPYKIDSKQASLLGDANIHGH TESMISAEL |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ROR1重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Structural and functional characterization of the ROR1 ectodomain based on a stable monomeric form*
**作者**：Huang, X., et al.
**摘要**：该研究通过哺乳动物表达系统成功表达并纯化重组人ROR1胞外域蛋白，发现其以单体形式稳定存在。通过X射线晶体学解析了ROR1的免疫球蛋白样结构域，揭示了其与Wnt5a结合的潜在表位，为靶向ROR1的抗体药物设计提供了结构基础。
2. **文献名称**：*Recombinant ROR1 protein production and its application in CAR-T cell therapy for solid tumors*
**作者**：Zheng, Y., et al.
**摘要**：研究利用昆虫细胞表达系统制备重组ROR1蛋白，并基于此开发了靶向ROR1的CAR-T细胞。体外实验表明，该CAR-T细胞能有效识别并杀伤ROR1高表达的乳腺癌和肺癌细胞系，为实体瘤免疫治疗提供了新策略。
3. **文献名称**：*ROR1-targeted antibody-drug conjugate development using recombinant ROR1-Fc fusion protein*
**作者**：Cui, B., et al.
**摘要**：通过将ROR1胞外域与人IgG1 Fc片段融合表达，获得重组ROR1-Fc蛋白，并用于筛选高亲和力单克隆抗体。基于此开发的抗体-药物偶联物(ADC)在慢性淋巴细胞白血病(CLL)小鼠模型中显著抑制肿瘤生长，验证了ROR1作为治疗靶点的潜力。
4. **文献名称**：*ROR1 recombinant protein reveals its role in non-canonical Wnt signaling pathway activation*
**作者**：Baskar, S., et al.
**摘要**：通过重组ROR1蛋白的体外功能实验，证实其与Wnt5a结合后可激活下游非经典Wnt信号通路(如JNK和Ca²⁺通路)，促进肿瘤细胞迁移和侵袭。研究进一步通过基因敲除验证了ROR1在肿瘤转移中的关键作用。
**注**：以上文献信息为示例性概括，具体内容需根据实际文献调整。建议通过PubMed或Google Scholar以“ROR1 recombinant protein”为关键词检索最新研究。
**Background of ROR1 Recombinant Protein**
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is a transmembrane protein belonging to the ROR family of receptors, which play critical roles in embryonic development, cell polarity, and migration. Unlike its homolog ROR2. ROR1 is minimally expressed in healthy adult tissues but is frequently overexpressed in various malignancies, including chronic lymphocytic leukemia (CLL), breast cancer, lung cancer, and pancreatic cancer. This tumor-specific expression profile has positioned ROR1 as a promising therapeutic target and biomarker for cancer diagnosis and treatment.
ROR1 recombinant protein is engineered in vitro using expression systems such as mammalian (e.g., HEK293 or CHO cells) or insect cells (e.g., Sf9) to mimic the native protein’s structure and function. The recombinant protein typically includes key domains of ROR1. such as the extracellular immunoglobulin (Ig)-like, cysteine-rich, and kringle domains, which mediate ligand binding and downstream signaling. Researchers often purify these proteins with tags (e.g., Fc or His tags) to facilitate detection, stabilization, or binding assays.
The primary applications of ROR1 recombinant protein span basic research and drug development. It is used to study ROR1’s interaction with ligands like Wnt-5a, its role in oncogenic pathways (e.g., NF-κB, PI3K/AKT), and its contribution to cancer cell survival, metastasis, and drug resistance. Additionally, the protein serves as an antigen for generating monoclonal antibodies or CAR-T cells targeting ROR1-positive cancers. Several ROR1-targeted therapies, such as cirmtuzumab (anti-ROR1 antibody) and related antibody-drug conjugates, are under clinical evaluation, highlighting the translational relevance of ROR1 recombinant tools.
Overall, ROR1 recombinant protein is a vital resource for unraveling the biology of ROR1-driven cancers and advancing targeted immunotherapies.
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