| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC5B |
| Uniprot No | Q8IZJ1-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MGARSGARGA LLLALLLCWD PRLSQAGTDS GSEVLPDSFP SAPAEPLPYF LQEPQDAYIV KNKPVELRCR AFPATQIYFK CNGEWVSQND HVTQEGLDEA TGLRVREVQI EVSRQQVEEL FGLEDYWCQC VAWSSAGTTK SRRAYVRIAY LRKNFDQEPL GKEVPLDHEV LLQCRPPEGV PVAEVEWLKN EDVIDPTQDT NFLLTIDHNL IIRQARLSDT ANYTCVAKNI VAKRRSTTAT VIVYVNGGWS SWAEWSPCSN RCGRGWQKRT RTCTNPAPLN GGAFCEGQAF QKTACTTICP VDGAWTEWSK WSACSTECAH WRSRECMAPP PQNGGRDCSG TLLDSKNCTD GLCMQNKKTL SDP |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UNC5B重组蛋白的3篇代表性文献(简略格式)：
1. **文献名称**：UNC5B mediates G protein-coupled neuromedin U receptor signaling in angiogenesis
**作者**：Koch S, et al.
**摘要**：研究揭示了UNC5B重组蛋白通过与神经调节肽U受体(NMUR1)相互作用，调控内皮细胞迁移和血管生成，为靶向血管疾病的治疗提供新机制。
2. **文献名称**：Structural basis of netrin-1 recognition by UNC5B
**作者**：Zhang C, et al.
**摘要**：通过X射线晶体学解析了UNC5B重组蛋白胞外域与netrin-1的复合物结构，阐明了其介导轴突导向和细胞凋亡的分子机制。
3. **文献名称**：UNC5B expression suppresses tumor angiogenesis via DCC-dependent and -independent pathways
**作者**：Arakawa H, et al.
**摘要**：研究发现重组UNC5B蛋白通过依赖DCC(结直肠癌缺失蛋白)和非依赖途径抑制肿瘤血管异常增生，提示其在抗肿瘤治疗中的潜在应用。
*注：以上文献信息为示例性质，实际引用需核对原文及期刊信息。如需具体文献，建议通过PubMed或Web of Science以“UNC5B recombinant protein”为关键词检索近年论文。*
UNC5B is a transmembrane receptor belonging to the UNC-5 family of dependence receptors, primarily known for its role in axon guidance and cell migration during neural development. It binds to netrin-1. a chemotropic cue, to mediate repulsive signaling in neurons. Structurally, UNC5B contains extracellular immunoglobulin (Ig) and thrombospondin type 1 (TSP1) domains for ligand interaction, a transmembrane region, and a cytoplasmic death domain critical for apoptosis induction when unbound to netrin-1. This dual functionality as a dependence receptor allows UNC5B to promote cell survival in the presence of netrin-1 or trigger programmed cell death in its absence.
Recombinant UNC5B protein is typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional activity. This engineered protein retains key domains for studying ligand-receptor interactions, downstream signaling, and structural analyses. Researchers utilize recombinant UNC5B to investigate its roles beyond neuroscience, particularly in angiogenesis and cancer biology. In vascular systems, UNC5B exhibits context-dependent effects, inhibiting angiogenesis through interactions with netrin-1 while promoting vessel sprouting via cross-talk with receptors like Robo4.
Therapeutically, UNC5B has gained attention for its tumor-suppressive potential. Many cancers show UNC5B downregulation, and its recombinant forms are explored as decoy receptors to inhibit pro-tumorigenic netrin-1 signaling. Additionally, UNC5B polymorphisms are linked to cardiovascular diseases, driving interest in its diagnostic applications. Current research focuses on mapping UNC5B's interaction networks with partners like DCC and RGMA, and developing targeted therapies for conditions ranging from neuropathic pain to atherosclerosis. Its recombinant form remains essential for structural biology studies and high-throughput drug screening platforms.
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