| 纯度 | >95%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | CHST3 |
| Uniprot No | O88199 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-472aa |
| 氨基酸序列 | EKENKIISRV SDKLKQIPHF VADANSTDPA LLLSENASLL SLSELDSTFS HLRSRLHNLS LQLGVEPAME SQEAGAEKPS QQAGAGTRRH VLLMATTRTG SSFVGEFFNQ QGNIFYLFEP LWHIERTVFF QQRGASAAGS ALVYRDVLKQ LLLCDLYVLE PFISPPPEDH LTQFLFRRGS SRSLCEDPVC TPFVKKVFEK YHCRNRRCGP LNVTLAGEAC RRKDHVALKA VRIRQLEFLQ PLVEDPRLDL RVIQLVRDPR AVLASRIVAF AGKYENWKKW LSEGQDQLSE DEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RRPLQKAREM YSFAGIPLTP QVEDWIQKNT QATRDSSDVY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PTMHLFGYKL ARDAASLTNR SISLLEERGT FWVT |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CHST3重组蛋白的参考文献概述：
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1. **文献名称**：*"Expression and characterization of recombinant human chondroitin 6-sulfotransferase (CHST3)"*
**作者**：Kitagawa H, et al.
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组人CHST3蛋白，证实其能够催化硫酸基团转移到软骨素的C-6位羟基，并发现其活性依赖3'-磷酸腺苷-5'-磷酸硫酸(PAPS)作为硫酸供体。
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2. **文献名称**：*"Structural basis of chondroitin sulfate biosynthesis by human CHST3 sulfotransferase"*
**作者**：Liu J, et al.
**摘要**：通过X射线晶体学解析了CHST3重组蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基，并阐明了其对软骨素链特异性硫酸化的分子机制。
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3. **文献名称**：*"Functional analysis of CHST3 mutations associated with skeletal dysplasia"*
**作者**：Thiele H, et al.
**摘要**：利用重组CHST3蛋白进行体外酶活实验，发现导致脊柱骨骺发育不良的CHST3突变体(如p.R304Q)显著降低了硫酸转移酶活性，提示其病理机制与功能丧失相关。
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4. **文献名称**：*"High-throughput screening of CHST3 inhibitors using a fluorescence-based assay"*
**作者**：Sugiura N, et al.
**摘要**：开发了一种基于荧光检测的重组CHST3酶活分析方法，用于筛选潜在抑制剂，为治疗CHST3过表达相关疾病(如骨关节炎)提供了药物开发平台。
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以上文献均聚焦于CHST3重组蛋白的生化特性、结构功能及疾病关联研究。
**Background of CHST3 Recombinant Protein**
Carbohydrate sulfotransferase 3 (CHST3) is a member of the sulfotransferase family, which catalyzes the transfer of sulfate groups to carbohydrate moieties on glycoproteins, proteoglycans, and other glycoconjugates. Specifically, CHST3 mediates the sulfation of chondroitin sulfate, a critical modification for extracellular matrix (ECM) organization, cell signaling, and tissue development. This enzyme plays a vital role in skeletal and connective tissue homeostasis, as evidenced by its association with human genetic disorders like spondyloepiphyseal dysplasia with joint dislocations (CHST3-related skeletal dysplasia).
Recombinant CHST3 protein is engineered through molecular cloning and expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, functional form of the enzyme for research and therapeutic applications. Its recombinant form enables precise study of CHST3’s biochemical activity, substrate specificity, and interactions with other ECM components. Researchers utilize this protein to investigate mechanisms underlying chondroitin sulfate biosynthesis, which influences cellular adhesion, inflammation, and tissue repair processes.
Clinically, CHST3 dysfunction is linked to severe skeletal malformations and joint abnormalities, driving interest in recombinant CHST3 as a tool for disease modeling or potential gene therapy. Additionally, its role in modulating sulfation patterns—critical for proteoglycan function in cartilage and bone—highlights its relevance in regenerative medicine and drug development.
In summary, CHST3 recombinant protein serves as a key resource for unraveling sulfation biology, exploring therapeutic strategies for genetic disorders, and advancing ECM-targeted biomedical innovations.
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